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PON1 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 18155-1-AP

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Con: 23 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

PON1 Antibody WB mouse liver tissue 18155-1-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 18155-1-AP(PON1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PON1 Antibody IHC human liver tissue 18155-1-AP

Immunohistochemical of paraffin-embedded human liver using 18155-1-AP(PON1 antibody) at dilution of 1:50 (under 10x lens)

PON1 Antibody IHC human liver tissue 18155-1-AP

Immunohistochemical of paraffin-embedded human liver using 18155-1-AP(PON1 antibody) at dilution of 1:50 (under 40x lens)

PON1 Antibody IP mouse liver tissue 18155-1-AP

IP Result of anti-PON1 (IP:18155-1-AP, 4ug; Detection:18155-1-AP 1:500) with mouse liver tissue lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse liver tissue, human plasma tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PON1 fusion protein Ag12934
Full name:
paraoxonase 1
Calculated molecular weight:
355aa,40 kDa
Observed molecular weight:
42-45 kDa
GenBank accession number:
BC074719
Gene ID (NCBI):
5444
Gene symbol
PON1
Synonyms
A esterase 1, Aromatic esterase 1, ESA, K 45, paraoxonase 1, PON, PON 1, PON1, Serum aryldialkylphosphatase 1

Background

PON1, also named as PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It can capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters. PON1 may mediate an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. For glycosylated, the MW of PON1 is migrated 42-45kd. 55kd is a dimmer of isoform CRA-b. The antibody has cross-reaction to PON2 and PON3.


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