PON1 Polyclonal antibody

PON1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 18155-1-AP

Synonyms

A esterase 1, Aromatic esterase 1, ESA, K 45, paraoxonase 1, PON, PON 1, PON1, Serum aryldialkylphosphatase 1



Tested Applications

Positive WB detected inmouse liver tissue, human plasma tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18155-1-AP targets PON1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PON1 fusion protein Ag12934
Full Name paraoxonase 1
Calculated molecular weight 355 aa, 40 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC074719
Gene symbol PON1
Gene ID (NCBI) 5444
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PON1, also named as PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It can capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters. PON1 may mediate an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. For glycosylated, the MW of PON1 is migrated 42-45kd. 55kd is a dimmer of isoform CRA-b. The antibody has cross-reaction to PON2 and PON3.

Protocols

Product Specific Protocols
WB protocol for PON1 antibody 18155-1-APDownload protocol
IHC protocol for PON1 antibody 18155-1-APDownload protocol
IP protocol for PON1 antibody 18155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Biochim Biophys Acta Mol Cell Biol Lipids

Paraoxonase 1 in endothelial cells impairs vasodilation induced by arachidonic acid lactone metabolite.

Authors - Dan Gilad
humanWB

Cell Death Dis

GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.

Authors - Qingcai Meng