PPA2 Polyclonal antibody

PPA2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16662-1-AP

Synonyms

HSPC124, PPA2, PPase 2, pyrophosphatase (inorganic) 2, Pyrophosphatase SID6 306, SID6 306



Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse liver tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16662-1-AP targets PPA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPA2 fusion protein Ag10138
Full Name pyrophosphatase (inorganic) 2
Calculated Molecular Weight305aa,35 kDa; 334aa,38 kDa
Observed Molecular Weight 32-40 kDa
GenBank Accession NumberBC057219
Gene Symbol PPA2
Gene ID (NCBI) 27068
RRIDAB_1851549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

Protocols

Product Specific Protocols
WB protocol for PPA2 antibody 16662-1-APDownload protocol
IHC protocol for PPA2 antibody 16662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols