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PPARD Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10156-2-AP

Featured Product KD/KO validated

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Con: 23 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

PPARD Antibody WB A549 cells 10156-2-AP

WB result of PPARD antibody (10156-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPARD transfected A549 cells

PPARD Antibody WB mouse heart tissue 10156-2-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP(PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

PPARD Antibody IHC human ovary tumor tissue 10156-2-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10156-2-AP(PPARD antibody) at dilution of 1:10 (under 10x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, COLO 320 cells, mouse skeletal muscle tissue, A549 cells
Positive IHC detected in:
human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PPARD fusion protein Ag0208
Full name:
peroxisome proliferator-activated receptor delta
Calculated molecular weight:
50 kDa
Observed molecular weight:
54 kDa
GenBank accession number:
BC002715
Gene ID (NCBI):
5467
Gene symbol
PPARD
Synonyms
FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

Background

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.


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