PPARD Polyclonal antibody
PPARD Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 10156-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, COLO 320 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
10156-2-AP targets PPARD in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PPARD fusion protein Ag0208 |
| Full Name | peroxisome proliferator-activated receptor delta |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC002715 |
| Gene symbol | PPARD |
| Gene ID (NCBI) | 5467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PPARD antibody 10156-2-AP | Download protocol |
| IHC protocol for PPARD antibody 10156-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chem Biol Interact Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy. | ||
J Adv Res Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling. | ||
Cell Death Dis Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/β-catenin/PPARδ signalling. | ||
Biochem Biophys Res Commun Naringenin exhibits the protective effect on cardiac hypertrophy via EETs-PPARs activation in streptozocin-induced diabetic mice. | ||
Biochem Biophys Res Commun Oleic acid up-regulates myosin heavy chain (MyHC) 1 expression and increases mitochondrial mass and maximum respiration in C2C12 myoblasts. |
