PRKAR1A Polyclonal antibody

PRKAR1A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20358-1-AP

Synonyms

CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, PRKAR1A, Tissue specific extinguisher 1, TSE1



Tested Applications

Positive WB detected inHeLa cells, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20358-1-AP is PRKAR1A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKAR1A fusion protein Ag14216
Full Name protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
Calculated molecular weight 381 aa, 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC036285
Gene symbol PRKAR1A
Gene ID (NCBI) 5573
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.

Protocols

Product Specific Protocols
WB protocol for PRKAR1A antibody 20358-1-APDownload protocol
IHC protocol for PRKAR1A antibody 20358-1-APDownload protocol
IF protocol for PRKAR1A antibody 20358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols