PrP Antibody

PrP Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 12555-1-AP

Synonyms

ASCR, CD230, CJD, GSS, Major prion protein, prion, prion protein, PRIP, PRNP, PrP, PrP27 30, PrP33 35C, PrPc



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12555-1-AP targets PrP in FC, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PrP fusion protein Ag3257
Full Name prion protein
Calculated molecular weight 34 kDa
Observed molecular weight 30 kDa
GenBank accession number BC022532
Gene symbol PRNP
Gene ID (NCBI) 5621
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Prion protein (PRNP) is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Prion diseases are TSEs, attributed to conformational conversion of the cellular prion protein (PrPC) into an abnormal conformer that accumulates in the brain. The two isoforms, PrPC and PrPS, have the same primary amino acid sequence and only differ in conformation. While PrPC is composed of 42% α-helix and only 3% β-sheet, PrPSc is composed of 30% α-helix and 43% β-sheet. PrPC converts to its pathogenic isoform when the region corresponding to the residues 108-144 fold into β-sheets. PrPC is very soluble in detergents and easily digested by proteases while the PrPSc is insoluble in detergents and resistant to protease digestion. Prion diseases exist in infectious, sporadic, and genetic forms.

Protocols

Product Specific Protocols
WB protocol for PrP antibody 12555-1-APDownload protocol
IHC protocol for PrP antibody 12555-1-APDownload protocol
IP protocol for PrP antibody 12555-1-APDownload protocol
FC protocol for PrP antibody 12555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transfusion

Integrative genome analysis identified the KANNO blood group antigen as prion protein.

Authors - Yosuke Omae
mouse,humanWB,IP

Neuron

Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.

Authors - Frank F Heisler