PrP Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 12555-1-AP

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Freight/Packing

Con: 38 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse brain tissue, human brain tissue, rat brain tissue

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
human gliomas tissue, human gliomas tissue, human pancreas cancer tissue

Positive FC detected in:
SH-SY5Y cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
prion protein

Calculated molecular weight:
34 kDa

Observed molecular weight:
30 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PRNP

Synonyms
ASCR, CD230, CJD, GSS, Major prion protein, prion, prion protein, PRIP, PRNP, PrP, PrP27 30, PrP33 35C, PrPc
Background

Prion protein (PRNP) is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Prion diseases are TSEs, attributed to conformational conversion of the cellular prion protein (PrPC) into an abnormal conformer that accumulates in the brain. The two isoforms, PrPC and PrPS, have the same primary amino acid sequence and only differ in conformation. While PrPC is composed of 42% α-helix and only 3% β-sheet, PrPSc is composed of 30% α-helix and 43% β-sheet. PrPC converts to its pathogenic isoform when the region corresponding to the residues 108-144 fold into β-sheets. PrPC is very soluble in detergents and easily digested by proteases while the PrPSc is insoluble in detergents and resistant to protease digestion. Prion diseases exist in infectious, sporadic, and genetic forms.


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