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  • Featured Product
  • KD/KO Validated

PTBP1 Monoclonal antibody

PTBP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D6D8

Cat no : 67462-1-Ig

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTBP1 Monoclonal antibody (67462-1-Ig)

    WB analysis using 67462-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67462-1-Ig (PTBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PTBP1 Monoclonal antibody (67462-1-Ig)

    WB analysis of HeLa using 67462-1-Ig

    WB result of PTBP1 antibody (67462-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human colon cancer using 67462-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human pancreas cancer using 67462-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human pancreas cancer using 67462-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PTBP1 Monoclonal antibody (67462-1-Ig)

    IHC staining of human colon cancer using 67462-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67462-1-Ig (PTBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using PTBP1 Monoclonal antibody (67462-1-Ig)

    IF Staining of A375 using 67462-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 67462-1-Ig (PTBP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, LNCaP cells, HEK-293 cells. K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67462-1-Ig targets PTBP1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PTBP1 fusion protein Ag28404
Full Name polypyrimidine tract binding protein 1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC013694
Gene Symbol PTBP1
Gene ID (NCBI) 5725
RRIDAB_2882692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10.

Protocols

Product Specific Protocols
WB protocol for PTBP1 antibody 67462-1-IgDownload protocol
IHC protocol for PTBP1 antibody 67462-1-IgDownload protocol
IF protocol for PTBP1 antibody 67462-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Toxicol Sci

Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS

Authors - Jia Luo
  • KD Validated
View Article