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PTEN Polyclonal antibody

PTEN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22034-1-AP

Synonyms

10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1



Tested Applications

Positive WB detected inDU 145 cells, mouse thymus tissue, HeLa cells, mouse testis tissue, C6 cells, rat testis tissue
Positive IP detected inDU 145 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse brain tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22034-1-AP is PTEN Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTEN fusion protein Ag17274
Full Name phosphatase and tensin homolog
Calculated molecular weight 47 kDa
Observed molecular weight 55 kDa, 68 kDa
GenBank accession numberBC005821
Gene symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatase and tensin homolog (PTEN) is an enzyme key in cell cycle regulation and in tumor suppression.


What is the molecular weight of PTEN?

PTEN is composed of 403 amino acid residues with an N-terminal domain with phosphatase activity and a 

C-terminal C2 domain that binds phospholipid membranes (PMID:14749127). This antibody can recognize 

two isoforms with MWs of 55kDa and 68 kDa.


What is the function of PTEN?

Widely expressed throughout the body, PTEN is found in the cytoplasm and inhibits cell cycle progression 

through phosphatase activity. It regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (PIP3) 

by catalyzing the dephosphorylation of the inositol ring. This function counteracts the growth-stimulating 

activity of the PI3K/AKT signaling pathway and thereby suppresses proliferation. Therefore, in homeostasis, 

PTEN promotes chromosome stability and DNA repair (PMID: 24387334).Expression and function can be 

regulated by posttranslational modifications, including acetylation for decreased catalytic activity and 

ubiquitination for protein degradation, nuclear localization, and inhibition of phosphatase activity 

(PMID: 24656806).


What is the role of PTEN in cancer?

PTEN was first identified as a tumor suppressor when mutations or deletions were identified by two separate 

groups in a number of different cancer types including gliomas, breast, prostate, and kidney cancer 

(PMID: 9090379; PMID: 9072974). Non-inherited, or somatic, mutations in PTEN that lead to a loss of function 

in the protein have been shown to drive malignancy in tumors. PTEN hamartoma tumor syndrome (PHTS) is a 

group of inherited disorders that occur as a result of autosomal dominant mutations in the PTEN gene. These 

disorders include Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. Because of 

the constitutive expression of PTEN, the symptoms are varied but often include neurodevelopmental issues and 

an increased risk of developing tumors, both benign and malignant (PMID: 26564076).


Protocols

Product Specific Protocols
WB protocol for PTEN antibody 22034-1-APDownload protocol
IHC protocol for PTEN antibody 22034-1-APDownload protocol
IP protocol for PTEN antibody 22034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biochem Biophys Res Commun

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Authors - Xi Shen
humanWB

Biosci Rep

Down-regulation of miR-543 expression increases the sensitivity of colorectal cancer cells to 5-Fluorouracil through the PTEN/PI3K/AKT pathway.

Authors - Gang Liu
humanIHC

Cancer Med

The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy.

Authors - Zhi Cao
humanWB

Onco Targets Ther

Hypoxic non-small-cell lung cancer cell-derived exosomal miR-21 promotes resistance of normoxic cell to cisplatin.

Authors - Caijun Dong
humanWB

Cancer Chemother Pharmacol

A panel of serum exosomal microRNAs as predictive markers for chemoresistance in advanced colorectal cancer.

Authors - Guoying Jin
humanWB,IHC

Biomed Res Int

PMID: 31011573

Authors - Qing Ma