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PTEN Antibody 36 Publications

Rabbit Polyclonal| Catalog number: 22034-1-AP

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Con: 46 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

PTEN Antibody WB DU 145 cells 22034-1-AP

DU 145 cells were subjected to SDS PAGE followed by western blot with 22034-1-AP( PTEN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

PTEN Antibody WB MKN-45 cells 22034-1-AP

MKN-45 cells were subjected to SDS PAGE followed by western blot with 22034-1-AP( PTEN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

PTEN Antibody IP DU 145 cells 22034-1-AP

IP result of anti-PTEN(IP:22034-1-AP, 4ug; Detection:22034-1-AP 1:500) with DU 145 cells lysate 1800 ug

PTEN Antibody IHC human prostate cancer tissue 22034-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP( PTEN Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PTEN Antibody IHC human prostate cancer tissue 22034-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP( PTEN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
DU 145 cells, mouse testis tissue, HeLa cells, MKN-45 cells, mouse thymus tissue
Positive IP detected in:
DU 145 cells
Positive IHC detected in:
human prostate cancer tissue, human breast cancer tissue, human colon cancer tissue, human testis tissue, mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PTEN fusion protein Ag17274
Full name:
phosphatase and tensin homolog
Calculated molecular weight:
47 kDa
Observed molecular weight:
55 kDa, 68 kDa
GenBank accession number:
BC005821
Gene ID (NCBI):
5728
Gene symbol
PTEN
Synonyms
10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Background

Phosphatase and tensin homolog (PTEN) is an enzyme key in cell cycle regulation and in tumor suppression.


What is the molecular weight of PTEN?

PTEN is composed of 403 amino acid residues with an N-terminal domain with phosphatase activity and a 

C-terminal C2 domain that binds phospholipid membranes (PMID:14749127). This antibody can recognize 

two isoforms with MWs of 55kDa and 68 kDa.


What is the function of PTEN?

Widely expressed throughout the body, PTEN is found in the cytoplasm and inhibits cell cycle progression 

through phosphatase activity. It regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate (PIP3) 

by catalyzing the dephosphorylation of the inositol ring. This function counteracts the growth-stimulating 

activity of the PI3K/AKT signaling pathway and thereby suppresses proliferation. Therefore, in homeostasis, 

PTEN promotes chromosome stability and DNA repair (PMID: 24387334).Expression and function can be 

regulated by posttranslational modifications, including acetylation for decreased catalytic activity and 

ubiquitination for protein degradation, nuclear localization, and inhibition of phosphatase activity 

(PMID: 24656806).


What is the role of PTEN in cancer?

PTEN was first identified as a tumor suppressor when mutations or deletions were identified by two separate 

groups in a number of different cancer types including gliomas, breast, prostate, and kidney cancer 

(PMID: 9090379; PMID: 9072974). Non-inherited, or somatic, mutations in PTEN that lead to a loss of function 

in the protein have been shown to drive malignancy in tumors. PTEN hamartoma tumor syndrome (PHTS) is a 

group of inherited disorders that occur as a result of autosomal dominant mutations in the PTEN gene. These 

disorders include Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and Proteus syndrome. Because of 

the constitutive expression of PTEN, the symptoms are varied but often include neurodevelopmental issues and 

an increased risk of developing tumors, both benign and malignant (PMID: 26564076).



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