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PTEN Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 22034-1-AP

Featured Product KD/KO validated

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Con: 46 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

PTEN Antibody WB DU 145 cells 22034-1-AP

DU 145 cells were subjected to SDS PAGE followed by western blot with 22034-1-AP( PTEN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

PTEN Antibody WB MKN-45 cells 22034-1-AP

MKN-45 cells were subjected to SDS PAGE followed by western blot with 22034-1-AP( PTEN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

PTEN Antibody IP DU 145 cells 22034-1-AP

IP result of anti-PTEN(IP:22034-1-AP, 4ug; Detection:22034-1-AP 1:500) with DU 145 cells lysate 1800 ug

PTEN Antibody IHC human prostate cancer tissue 22034-1-AP

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP( PTEN Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PTEN Antibody IHC human prostate cancer tissue 22034-1-AP

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 22034-1-AP( PTEN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PTEN Antibody IF HeLa cells 22034-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 22034-1-AP(PTEN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
DU 145 cells, mouse testis tissue, HeLa cells, MKN-45 cells, mouse thymus tissue
Positive IP detected in:
DU 145 cells
Positive IHC detected in:
human prostate cancer tissue, human breast cancer tissue, human colon cancer tissue, human testis tissue, mouse brain tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PTEN fusion protein Ag17274
Full name:
phosphatase and tensin homolog
Calculated molecular weight:
47 kDa
Observed molecular weight:
55 kDa, 68 kDa
GenBank accession number:
BC005821
Gene ID (NCBI):
5728
Gene symbol
PTEN
Synonyms
10q23del, Bannayan Zonana syndrome, BC005821, BZS, ITGA 2, MGC11227, MHAM, MMAC1, Multiple hamartoma (Cowden syndrome), phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Background

PTEN(Phosphatase and tensin homolog) is also named as MMAC1, TEP1 and it modulates cell cycle progression and cell survival (blockage) through down-regulating the positive cell cycle-regulator (cycle D1) by its phosphatase activity and up-regulating the negative cell cycle regulator p21 (by its lipid phosphatase activity). PTEN is an important tumor suppressor whose inactivation frequency in cancers is ranked second after p53 tumor suppressor inactivation. PTEN is a 403-residue protein structured in an amino (N)-terminal phosphatase domain and a carboxy (C)-terminal C2 domain that binds phospholipid membranes.(PMID:14749127). There are some report showing that SUMOylation appears to be a positive regulator in controlling PTEN membrane association, whereas phosphorylation is a negative regulator that may neutralize SUMOylation through intramolecular electrostatic interactions (PMID:22713753). This antibody can recognize two isoforms with MW of 55kDa and 68 kDa.


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