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COX2 Antibody 82 Publications

Rabbit Polyclonal| Catalog number: 12375-1-AP

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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COX2 Antibody WB LPS treated HEK-293 12375-1-AP

LPS treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12375-1-AP(PTGS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

COX2 Antibody IHC human lung cancer tissue 12375-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12375-1-AP( COX2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

COX2 Antibody IHC human lung cancer tissue 12375-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12375-1-AP( COX2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

COX2 Antibody IF HepG2 cells 12375-1-AP

Immunofluorescent analysis of HepG2 cells using 12375-1-AP(COX2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse, rat, chicken
Positive WB detected in:
LPS treated HEK-293, RAW264.7, HEK-293 cells, LPS treated RAW264.7, mouse embryo tissue, rat stomach tissue
Positive IHC detected in:
human lung cancer tissue, human placenta tissue, human testis tissue, human ovary tissue, human colon cancer tissue, human prostate hyperplasia tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
COX2 fusion protein Ag3025
Full name:
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight:
604aa,68 kDa
Observed molecular weight:
70-74 kDa, 64 kDa
GenBank accession number:
BC013734
Gene ID (NCBI):
5743
Gene symbol
PTGS2
Synonyms
COX 2, COX2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

Background

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.


1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72–74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).


Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

COX2 Rabbit Polyclonal antibody

Ryan (Verified Customer), 02-28-2019
Applications
Immunohistochemistry,Immunofluorescence,
Primary Antibody Dilution
1:100
COX2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (microglia) at 3dpf (Cat no:12375-1-AP)

COX2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (microglia) at 3dpf (Cat no:12375-1-AP)

Cell/Tissue Type
Zebrafish brain (microglia) at 3dpf
Lot Number
Tissue was fixed in PFA and no further antigen retrieval was necessary. Co-localisation in microglia shown with a known marker (magenta).

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