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- KD/KO Validated
COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody
COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
181
Cat no : 12375-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells, LPS treated HEK-293, RAW264.7, HEK-293 cells, LPS treated RAW264.7, rat stomach tissue, mouse embryo tissue, Raji cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human colon cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 163 publications below |
| IHC | See 21 publications below |
| IF | See 4 publications below |
Product Information
12375-1-AP targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | chicken, human, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag3025 |
| Full Name | prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) |
| Calculated molecular weight | 604 aa, 68 kDa |
| Observed molecular weight | 70-74 kDa, 64 kDa |
| GenBank accession number | BC013734 |
| Gene symbol | PTGS2 |
| Gene ID (NCBI) | 5743 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.
1. What is the molecular weight of COX2?
The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also
expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented
PTGS2 has also previously been detected in AD brains (PMID:14724276).
2. Is COX2 post-translationally modified?
COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and
s-nitrosylation (PMID: 28939645).
3. What is the difference between COX1, COX2, and COX3?
There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1
is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly
induced by various stimuli (as part of infection and inflammatory response) and is controlled by the
transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered
non-functional in humans.
4. I cannot detect COX2 in my sample during western blotting.
Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines,
and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally,
the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels
(PMID: 10966456), which should be taken into account during experimental design.
5. What is the role of COX2 in cancer?
COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with
greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.
6. What is subcellular localization of COX2?
Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330),
but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-AP | Download protocol |
| IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-AP | Download protocol |
| IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 12375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Iran J Basic Med Sci Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress. | ||
Biomed Res Int Rapamycin and FTY720 Alleviate Atherosclerosis by Cross Talk of Macrophage Polarization and Autophagy. | ||
Int Immunopharmacol Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice. | ||
Oxid Med Cell Longev Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice. | ||
Molecules In Vitro and In Vivo Anti-Inflammatory Effects of Polyphyllin VII through Downregulating MAPK and NF-κB Pathways. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ryan (Verified Customer) (02-28-2019) | Tissue was fixed in PFA and no further antigen retrieval was necessary. Co-localisation in microglia shown with a known marker (magenta).
 |
