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  • Featured Product
  • KD/KO Validated

PUM1 Polyclonal antibody

PUM1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26256-1-AP

Synonyms

HSPUM, KIAA0099, PUM1, PUMH, PUMH1, Pumilio 1, Pumilio homolog 1, pumilio homolog 1 (Drosophila), PUML1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PUM1 Polyclonal antibody (26256-1-AP)

    WB analysis of MCF-7 using 26256-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26256-1-AP (PUM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PUM1 Polyclonal antibody (26256-1-AP)

    IP experiment of MCF-7 using 26256-1-AP

    IP result of anti-PUM1 (IP:26256-1-AP, 4ug; Detection:26256-1-AP 1:300) with MCF-7 cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PUM1 Polyclonal antibody (26256-1-AP)

    IF Staining of MCF-7 using 26256-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26256-1-AP (PUM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inMCF-7 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26256-1-AP is PUM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PUM1 fusion protein Ag24651
Full Name pumilio homolog 1 (Drosophila)
Calculated Molecular Weight 1186 aa, 126 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC013398
Gene Symbol PUM1
Gene ID (NCBI) 9698
RRIDAB_2880448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PUM1, also named as Pumilio homolog 1, is a 1186 amino acid protein, which is expressed in brain, heart, kidney, muscle, intestine and stomach. PUM1 as sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). The calculated molecular weight of PUM1 is 126 kDa, but the modified PUM1 is about 130-140 kDa.

Protocols

Product Specific Protocols
WB protocol for PUM1 antibody 26256-1-APDownload protocol
IF protocol for PUM1 antibody 26256-1-APDownload protocol
IP protocol for PUM1 antibody 26256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biochem Biophys Res Commun

PUM1 promotes ovarian cancer proliferation, migration and invasion.

Authors - Xue Guan
  • KD Validated
View Article
humanWB

Carcinogenesis

PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway.

Authors - Beatriz Escudero-Paniagua
View Article