Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 10029-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 10029-1-Ig
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (Paxillin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10029-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10029-1-Ig
MCF7 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10029-1-Ig
IP result of anti-Paxillin (IP:10029-1-Ig, 3ug; Detection:10029-1-Ig 1:1000) with mouse brain tissue lysate 7500ug.
IHC staining of human liver cancer using 10029-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 10029-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver cancer using 10029-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10029-1-Ig (Paxillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HUVEC using 10029-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Paxillin antibody (10029-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).
IF Staining of HepG2 using 10029-1-Ig
Immunofluorescent analysis of -20℃ Ethanol fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin). DAPI (blue).
IF Staining of HepG2 using 10029-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of NIH/3T3 using 10029-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 10029-1-Ig(Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Paxillin Polyclonal antibody

Name: Paxillin Polyclonal antibody
Brand: Proteintech
SKU: 10029-1-Ig
Rating: 4.50 (2 reviews)

Paxillin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10029-1-Ig

Publications(20)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PXN

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (20)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, COLO 320 cells, Jurkat cells, MCF-7 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HUVEC cells, NIH/3T3 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 3 publications below
IF	See 9 publications below

Product Information

10029-1-Ig targets Paxillin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein Predict reactive species
Full Name	paxillin
Calculated Molecular Weight	68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	NM_002859
Gene Symbol	Paxillin
Gene ID (NCBI)	5829
RRID	AB_513929
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P49023
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).

Protocols

Product Specific Protocols
WB protocol for Paxillin antibody 10029-1-Ig	Download protocol
IHC protocol for Paxillin antibody 10029-1-Ig	Download protocol
IF protocol for Paxillin antibody 10029-1-Ig	Download protocol
IP protocol for Paxillin antibody 10029-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine. Authors - Daniel Arango View Article
rat	IHC	Sci Adv Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction Authors - Yuzhi Sun View Article
human	IF	Bone Res Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma Authors - Ming Luo View Article
human	IF	Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells Authors - Yingqi Liu View Article
human	WB	Cell Death Dis ZC3H15 promotes glioblastoma progression through regulating EGFR stability. Authors - Jianbing Hou View Article
human	IF	Acta Pharmacol Sin Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation. Authors - Kai Li View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Thomas (Verified Customer) (09-22-2022)

Works in WB (pulldown via PAK1) and IF (costained with F-Actin in cyan)

Applications: Western Blot, Immunofluorescence
Primary Antibody Dilution: 1:100 (IF), 1:500 (WB)
Cell Tissue Type: Human Brain Microvascular endothelial cell line

Paxillin Antibody Western Blot,Immunofluorescence validation (1:100 (IF), 1:500 (WB) dilution) in Human Brain Microvascular endothelial cell line (Cat no:10029-1-Ig)

Boyan (Verified Customer) (03-11-2019)

For WB, besides the expected band, it also recognised some other non-specific bands; for IF, it could label the cell cortex localisation, partially co-localised with Actin.