Paxillin Polyclonal antibody

Paxillin Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 10029-1-Ig


paxillin, PXN

Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, Jurkat cells, MCF-7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, NIH/3T3 cells, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10029-1-Ig targets Paxillin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name paxillin
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberNM_002859
Gene Symbol Paxillin
Gene ID (NCBI) 5829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).


Product Specific Protocols
WB protocol for Paxillin antibody 10029-1-IgDownload protocol
IHC protocol for Paxillin antibody 10029-1-IgDownload protocol
IF protocol for Paxillin antibody 10029-1-IgDownload protocol
IP protocol for Paxillin antibody 10029-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.

Authors - Daniel Arango

Bone Res

Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

Authors - Ming Luo

Cell Rep

Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells

Authors - Yingqi Liu

Cell Death Dis

ZC3H15 promotes glioblastoma progression through regulating EGFR stability.

Authors - Jianbing Hou

Acta Pharmacol Sin

Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.

Authors - Kai Li

Sci Signal

Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.

Authors - Ouranio Anastasiou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Thomas (Verified Customer) (09-22-2022)

Works in WB (pulldown via PAK1) and IF (costained with F-Actin in cyan)

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:100 (IF), 1:500 (WB)
  • Cell Tissue Type: Human Brain Microvascular endothelial cell line
Paxillin Antibody Western Blot,Immunofluorescence validation (1:100 (IF), 1:500 (WB) dilution) in Human Brain Microvascular endothelial cell line (Cat no:10029-1-Ig)

Boyan (Verified Customer) (03-11-2019)

For WB, besides the expected band, it also recognised some other non-specific bands; for IF, it could label the cell cortex localisation, partially co-localised with Actin.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: MEF