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  • Featured Product
  • KD/KO Validated

PYCR1 Polyclonal antibody

PYCR1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 13108-1-AP

Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PYCR1 Polyclonal antibody (13108-1-AP)

    WB analysis using 13108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PYCR1 Polyclonal antibody (13108-1-AP)

    WB analysis of HeLa cells using 13108-1-AP

    WB result of PYCR1 antibody (13108-1-AP, 1:5000) with si-Control and si-PYCR1 transfected HeLa cells.

  • Western Blot (WB) analysis of human brain tissue using PYCR1 Polyclonal antibody (13108-1-AP)

    WB analysis of human brain using 13108-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse embryo tissue using PYCR1 Polyclonal antibody (13108-1-AP)

    WB analysis of mouse embryo using 13108-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PYCR1 Polyclonal antibody (13108-1-AP)

    WB analysis of NIH/3T3 using 13108-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PYCR1 Polyclonal antibody (13108-1-AP)

    IP experiment of mouse brain using 13108-1-AP

    IP result of anti-PYCR1 (IP:13108-1-AP, 3ug; Detection:13108-1-AP 1:700) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PYCR1 Polyclonal antibody (13108-1-AP)

    IHC staining of human prostate cancer using 13108-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PYCR1 Polyclonal antibody (13108-1-AP)

    IHC staining of human prostate cancer using 13108-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PYCR1 Polyclonal antibody (13108-1-AP)

    IHC staining of human prostate cancer using 13108-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13108-1-AP (PYCR1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PYCR1 Polyclonal antibody (13108-1-AP)

    IF Staining of MCF-7 using 13108-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PYCR1 antibody (13108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PYCR1 Polyclonal antibody (13108-1-AP)

    IF Staining of MCF-7 using 13108-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PYCR1 antibody 13108-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inCOLO 320 cells, mouse embryo tissue, human brain tissue, HeLa cells, NIH/3T3 cells, HT-1080 cells，mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13108-1-AP targets PYCR1 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag3764
Full Name pyrroline-5-carboxylate reductase 1
Calculated Molecular Weight 319 aa, 33.8 kDa
Observed Molecular Weight 33 kDa, 35 kDa
GenBank Accession NumberBC022244
Gene Symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_2174878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR1 antibody 13108-1-APDownload protocol
IHC protocol for PYCR1 antibody 13108-1-APDownload protocol
IF protocol for PYCR1 antibody 13108-1-APDownload protocol
IP protocol for PYCR1 antibody 13108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nature

Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.

Authors - Fabricio Loayza-Puch
  • KO Validated
View Article
humanWB

Nature

Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.

Authors - Possemato Richard R
View Article
zebrafishWB,IF

Nat Genet

Mutations in PYCR1 cause cutis laxa with progeroid features.

Authors - Reversade Bruno B
View Article
mouseWB

Cell Metab

Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.

Authors - Sukru Anil Dogan
View Article
humanIHC

J Hepatol

Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.

Authors - Zhaobing Ding
View Article
human,mouseWB,IF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Daniel (Verified Customer) (02-05-2020)

The antibody stained the glomerulus of the mouse kidney.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney
PYCR1 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:13108-1-AP)