Phospho-P53 (Ser15) Polyclonal antibody
Phospho-P53 (Ser15) Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 28961-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | etoposide treated HT-29 cells |
| Positive IF detected in | HT-29 cells, etoposide treated HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 19 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
28961-1-AP targets Phospho-P53 (Ser15) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | tumor protein p53 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC003596 |
| Gene Symbol | TP53 |
| Gene ID (NCBI) | 7157 |
| RRID | AB_2881236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-P53 (Ser15) antibody 28961-1-AP | Download protocol |
| IF protocol for Phospho-P53 (Ser15) antibody 28961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation | ||
Acta Pharmacol Sin Exosomes are involved in total body irradiation-induced intestinal injury in mice. | ||
J Nutr Biochem Lycopene suppresses gastric cancer cell growth without affecting normal gastric epithelial cells | ||
Clin Epigenetics Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line | ||
Apoptosis High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines. | ||
J Biol Chem Vibrio splendidus flagellin C binds tropomodulin to induce p38 MAPK-mediated p53-dependent coelomocyte apoptosis in Echinodermata. |
