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  • KD/KO Validated

QSOX1 Polyclonal antibody

QSOX1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12713-1-AP

Synonyms

hQSOX, Q6, QSCN6, QSOX1, Quiescin Q6, Sulfhydryl oxidase 1



Tested Applications

Positive WB detected inSW 1990 cells, HeLa cells, HT-1080 cells, human heart tissue, human placenta tissue, MCF-7 cells, mouse heart tissue
Positive IP detected inSW 1990 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12713-1-AP targets QSOX1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen QSOX1 fusion protein Ag3396
Full Name quiescin Q6 sulfhydryl oxidase 1
Calculated molecular weight 747 aa, 83 kDa
Observed molecular weight 65-94 kDa
GenBank accession numberBC017692
Gene symbol QSOX1
Gene ID (NCBI) 5768
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

QSOX1(Sulfhydryl oxidase 1) catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.It has two isoforms with the molecular weight of 67 kda and 83 kDa,respectively. It is detected a band of 95 Kda in HT1080 cell lines (PMID:17331072).Isoform 1 is expressed on golgi apparatus membrane and isoform 2 is secreted (PMID:10708601). The isoform1(QSOX1a) is the full length of QSOX1, approximately 78 kd; and the isoform2(QSOX1b) is the splicing form, approximately 62kd(PMID:14633699; 2496424). QSOX1 is highly expressed in tumor cell lines but is not expressed in adjacent normal cells (PMID:21989104).

Protocols

Product Specific Protocols
WB protocol for QSOX1 antibody 12713-1-APDownload protocol
IHC protocol for QSOX1 antibody 12713-1-APDownload protocol
IP protocol for QSOX1 antibody 12713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Oncol

A Preliminary Proteomic Investigation of Circulating Exosomes and Discovery of Biomarkers Associated with the Progression of Osteosarcoma in a Clinical Model of Spontaneous Disease.

Authors - Jacqueline V Brady
ratWB

J Neurochem

Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels.

Authors - Margaret E Tome
humanWB,IHC

Mol Cancer Res

Quiescin sulfhydryl oxidase 1 promotes invasion of pancreatic tumor cells mediated by matrix metalloproteinases.

Authors - Katchman Benjamin A BA
humanWB

Mol Biol Cell

Vitamin K epoxide reductase contributes to protein disulfide formation and redox homeostasis within the endoplasmic reticulum.

Authors - Rutkevich Lori A LA
  • KD Validated
humanWB,IHC

J Proteome Res

Analysis of the plasma peptidome from pancreas cancer patients connects a peptide in plasma to overexpression of the parent protein in tumors.

Authors - Antwi Kwasi K
hamsterWB,IF

ACS Synth Biol

Enhanced Biosynthesis Performance of Heterologous Proteins in CHO-K1 Cells Using CRISPR-Cas9.

Authors - Wenpeng Wang