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RAB11A-Specific Polyclonal antibody
RAB11A-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 20229-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293T cells, HeLa cells, human brain tissue, mouse colon tissue, PC-12 cells, rat brain tissue, SH-SY5Y cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, IMCD3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20229-1-AP targets RAB11A-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||RAB11A, member RAS oncogene family|
|Calculated molecular weight||24 kDa|
|Observed molecular weight||24 kDa|
|GenBank accession number||NM_004663|
|Gene ID (NCBI)||8766|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.
|Product Specific Protocols|
|WB protocol for RAB11A-Specific antibody 20229-1-AP||Download protocol|
|IHC protocol for RAB11A-Specific antibody 20229-1-AP||Download protocol|
|IF protocol for RAB11A-Specific antibody 20229-1-AP||Download protocol|
|IP protocol for RAB11A-Specific antibody 20229-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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