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RAB11A-Specific Polyclonal antibody

RAB11A-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20229-1-AP

Synonyms

Rab 11, RAB11, RAB11A, Ras related protein Rab 11A, YL8



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, human brain tissue, mouse colon tissue, PC-12 cells, rat brain tissue, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, IMCD3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20229-1-AP targets RAB11A-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB11A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberNM_004663
Gene symbol RAB11A
Gene ID (NCBI) 8766
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939). The antibody is specific to RAB11A, and has no cross reaction to RAB11B.

Protocols

Product Specific Protocols
WB protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
IHC protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
IF protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
IP protocol for RAB11A-Specific antibody 20229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R.

Authors - Kobina Essandoh

Methods Mol Biol

Visualizing Coronavirus Entry into Cells.

Authors - Aleksandra Milewska
canine,ratWB,IHC

J Histochem Cytochem

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

Authors - Hiroshi Gomi
humanIF

FASEB J

DIAPH1 regulates ciliogenesis and trafficking in primary cilia.

Authors - Oliva Palander
humanIF

Br J Pharmacol

An orally available hypoglycaemic peptide taken up by caveolae transcytosis displays improved hypoglycaemic effects and body weight control in db/db mice.

Authors - Weisheng Lu

J Cell Biol

BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.

Authors - William J Monis