RAB4A Antibody

RAB4A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 10347-1-AP

Synonyms

HRES 1/RAB4, RAB4, RAB4A, Ras related protein Rab 4A



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, PC-12 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10347-1-AP targets RAB4A in IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB4A fusion protein Ag0357
Full Name RAB4A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24-28 kDa
GenBank accession number BC002438
Gene symbol RAB4A
Gene ID (NCBI) 5867
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

The human RAB genes share structural and biochemical properties with the Ras gene superfamily, and RAB4 (synonym: RAB4A) is a small GTPase belonging to this Ras superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. RAB4 may also function as regulator along the receptor recycling pathway.

Protocols

Product Specific Protocols
WB protocol for RAB4A antibody 10347-1-APDownload protocol
IHC protocol for RAB4A antibody 10347-1-APDownload protocol
IF protocol for RAB4A antibody 10347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Pathog Dis

Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.

Authors - Jia-Hui Deng
mouseWB

FASEB J

Tsg101 positively regulates physiologic-like cardiac hypertrophy through FIP3-mediated endosomal recycling of IGF-1R.

Authors - Kobina Essandoh
humanWB

Virology

Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells.

Authors - Takeshi Yamasaki
mouseIF

MBio

Yersinia pestisTargets the Host Endosome Recycling Pathway during the Biogenesis of theYersinia-Containing Vacuole To Avoid Killing by Macrophages.

Authors - Michael G Connor

Nat Commun

CHML promotes liver cancer metastasis by facilitating Rab14 recycle.

Authors - Tian-Wei Chen