RAB6B Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 10340-1-AP

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Freight/Packing

Con: 20 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse brain tissue, C6 cells, rat brain tissue

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
human gliomas tissue

Positive IF detected in:
C6 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
RAB6B, member RAS oncogene family

Calculated molecular weight:
23 kDa

Observed molecular weight:
24 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
RAB6B

Synonyms
RAB6B, Ras related protein Rab 6B
Background

The human RAB genes share structural and biochemical properties with the Ras gene superfamily. Accumulating data suggests an important role for RAB proteins either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from endoplasmic reticulum to the Golgi complex and to secretory vesicles involves the movement of carrier vesicles, a process that appears to involve RAB protein function. Rab6A has been shown to be a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Rab6B is encoded by an independent gene which is located on chromosome 3 region q21-q23. In contrast to Rab6A whose expression is ubiquitous, Rab6B is expressed in a tissue and cell-type specific manner. Rab6B is predominantly expressed in brain and the neuroblastoma cells. In brain, Rab6B was found to be specifically expressed in microglia, pericytes and Purkinje cells. Endogenous Rab6B localises to the Golgi apparatus and to ERGIC-53-positive vesicles. Comparable studies between Rab6A and Rab6B revealed distinct biochemical and cellular properties. Rab6B displays lower GTP-binding activities and is distributed over Golgi and ER membranes, whereas Rab6A is more restricted to the Golgi apparatus. Since the GTP-bound form of Rab6B does interact with all known Rab6A effectors, including Rabkinesin-6, the results suggest a cell-type specific role for Rab6B in retrograde membrane traffic at the level of the Golgi complex.


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