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  • KD/KO Validated

RAB8A Polyclonal antibody

RAB8A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55296-1-AP

Synonyms

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, mouse skin tissue, mouse lung tissue, human brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55296-1-AP targets RAB8A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB8A, member RAS oncogene family
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberNM_005370
Gene symbol RAB8A
Gene ID (NCBI) 4218
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

Protocols

Product Specific Protocols
WB protocol for RAB8A antibody 55296-1-APDownload protocol
IHC protocol for RAB8A antibody 55296-1-APDownload protocol
IF protocol for RAB8A antibody 55296-1-APDownload protocol
IP protocol for RAB8A antibody 55296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

World J Surg Oncol

RAB8A a new biomarker for endometrial cancer?

Authors - Yachun Bie
humanWB,IF

Sci Rep

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Authors - Ying-Da Chen
  • KD Validated
humanIF

J Cell Sci

S-acylation regulates the trafficking and stability of the unconventional Q-SNARE STX19.

Authors - Khamal K Ampah
humanWB

Hum Mol Genet

Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.

Authors - Myung Jong Kim
humanWB

Hum Mol Genet

The Parkinson's disease-linked protein TMEM230 is required for Rab8a-mediated secretory vesicle trafficking and retromer trafficking.

Authors - Myung Jong Kim
  • KD Validated
humanIF

Sci Rep

The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis.

Authors - Fangyan Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sara (Verified Customer) (04-30-2020)

Unfortunately this antibody did not work for my samples. I followed all the instructions in the "WB protocol for Rab8a antibody 55296-1-AP" that Proteintech provides. I did not get almost any signal when following those instructions (primary antibody 1:600 for 1h at 30-35°C). After repeating the experiment incubating the membrane with 1:600 primary antibody O/N at 4°C I got better signal, but still couldn't see a band of 24 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:600
  • Cell Tissue Type: Primary B cells from mouse spleen