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RAB8A Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 55296-1-AP

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Con: 53 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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RAB8A Antibody WB HeLa cells 55296-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

RAB8A Antibody IP HeLa cells 55296-1-AP

IP Result of anti-RAB8A (IP:55296-1-AP, 3ug; Detection:55296-1-AP 1:500) with HeLa cells lysate 2500ug.

Species specificity:
human, mouse, rat, dog
Positive WB detected in:
HeLa cells, NIH/3T3 cells, HepG2 cells, mouse skin tissue, mouse lung tissue, human brain tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
RAB8A, member RAS oncogene family
Calculated molecular weight:
24 kDa
Observed molecular weight:
24 kDa
GenBank accession number:
NM_005370
Gene ID (NCBI):
4218
Gene symbol
RAB8A
Synonyms
MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

Background

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.


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