Tested Applications
| Positive WB detected in | A431 cells, mouse lung tissue, HepG2 cells, NIH/3T3 cells, human brain tissue, mouse skin tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55296-1-AP targets RAB8A in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | RAB8A, member RAS oncogene family |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | NM_005370 |
| Gene Symbol | RAB8A |
| Gene ID (NCBI) | 4218 |
| RRID | AB_10858398 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61006 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB8A antibody 55296-1-AP | Download protocol |
| IHC protocol for RAB8A antibody 55296-1-AP | Download protocol |
| IP protocol for RAB8A antibody 55296-1-AP | Download protocol |
| WB protocol for RAB8A antibody 55296-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB8A antibody (Cat# 55296-1-AP) has 36 total citations. Citations appear in journals including Nature Communications, PNAS, Cell Research, Immunity, Nature Cell Biology, Oncogene, Circulation Research, Journal of Cell Biology, and others. Research fields span ciliogenesis, vesicle trafficking and exocytosis, autophagy, cancer (breast, prostate, colorectal, endometrial), neurodegeneration, cardiovascular biology, metabolism, and immune signaling.
| Species | Application | Title |
|---|---|---|
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
Immunity Targeting intracellular oncoproteins with dimeric IgA promotes expulsion from the cytoplasm and immune-mediated control of epithelial cancers | ||
Nat Cell Biol A molecular network for de novo generation of the apical surface and lumen.
| ||
Nat Commun Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis. | ||
Nat Commun Identification of a component of the tubulin poly-glutamylase complex required for phosphoinositide homeostasis and cilium assembly and maintenance. | ||
Nat Commun Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice |
Reviews
What customers say
One reviewer rated this antibody 2/5 stars for Western Blot on primary mouse splenic B cells. Following the recommended protocol (1:600, 1h at 30–35°C), they observed almost no signal. After repeating with an overnight 4°C incubation at the same dilution, signal improved but the expected 24 kDa band was still not detected. Overall, the user found the antibody underperforming despite protocol compliance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sara (Verified Customer) (04-30-2020) | Unfortunately this antibody did not work for my samples. I followed all the instructions in the "WB protocol for Rab8a antibody 55296-1-AP" that Proteintech provides. I did not get almost any signal when following those instructions (primary antibody 1:600 for 1h at 30-35°C). After repeating the experiment incubating the membrane with 1:600 primary antibody O/N at 4°C I got better signal, but still couldn't see a band of 24 kDa.
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