Tested Applications
| Positive WB detected in | mouse testis tissue, COLO 320 cells |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 16 publications below |
| IP | See 2 publications below |
Product Information
13354-1-AP targets p107 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | p107 fusion protein Ag4146 Predict reactive species |
| Full Name | retinoblastoma-like 1 (p107) |
| Calculated Molecular Weight | 1068 aa, 119 kDa |
| Observed Molecular Weight | 119 kDa |
| GenBank Accession Number | BC032247 |
| Gene Symbol | p107 |
| Gene ID (NCBI) | 5933 |
| RRID | AB_2238024 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28749 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Retinoblastoma-like protein 1, also named RBL1, p107, is a member of E1A-binding proteins, which contains a A/B pocket, or pRb. Via this structure, RBL1 can bind to viral oncoproteins and other perteins containing LXCXE motif. It regulates cell proliferation via phosphorylation-sensitive interactions with E2F transcription factors and other proteins. Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.(uniprot). It has been reported there are two transcirpts variants of RBL1, which encode a 119-kd protein and a 68-kd protein(PMID:7490090).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p107 antibody 13354-1-AP | Download protocol |
| IP protocol for p107 antibody 13354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun p107 mediated mitochondrial function controls muscle stem cell proliferative fates. | ||
Cancers (Basel) RBL1/p107 Expression Levels Are Modulated by Multiple Signaling Pathways. | ||
Front Cell Dev Biol miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis | ||
Oncotarget Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells.
| ||
Biochem Pharmacol Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms. | ||
Mol Pharm Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells. |
