- Featured Product
- KD/KO Validated
p107 Polyclonal antibody
p107 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 13354-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, COLO 320 cells |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
Product Information
13354-1-AP targets p107 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | p107 fusion protein Ag4146 |
| Full Name | retinoblastoma-like 1 (p107) |
| Calculated molecular weight | 1068 aa, 119 kDa |
| Observed molecular weight | 119 kDa |
| GenBank accession number | BC032247 |
| Gene symbol | RBL1 |
| Gene ID (NCBI) | 5933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Retinoblastoma-like protein 1, also named RBL1, p107, is a member of E1A-binding proteins, which contains a A/B pocket, or pRb. Via this structure, RBL1 can bind to viral oncoproteins and other perteins containing LXCXE motif. It regulates cell proliferation via phosphorylation-sensitive interactions with E2F transcription factors and other proteins. Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.(uniprot). It has been reported there are two transcirpts variants of RBL1, which encode a 119-kd protein and a 68-kd protein(PMID:7490090).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p107 antibody 13354-1-AP | Download protocol |
| IP protocol for p107 antibody 13354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Mammalian Pum1 and Pum2 Control Body Size via Translational Regulation of the Cell Cycle Inhibitor Cdkn1b. | ||
Cancers (Basel) Loss of Rb1 Enhances Glycolytic Metabolism in Kras-Driven Lung Tumors In Vivo. | ||
Chem Biol Interact Panaxydol inhibits the proliferation and induces the differentiation of human hepatocarcinoma cell line HepG2. | ||
PLoS One Expression of the lncRNA Maternally Expressed Gene 3 (MEG3) Contributes to the Control of Lung Cancer Cell Proliferation by the Rb Pathway. | ||
Mol Pharm Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells. | ||
Biochem Pharmacol Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms. |
