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RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22335-1-AP

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCC1 Polyclonal antibody (22335-1-AP)

    WB analysis using 22335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RCC1 Polyclonal antibody (22335-1-AP)

    WB analysis of HepG2 using 22335-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22335-1-AP (RCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RCC1 Polyclonal antibody (22335-1-AP)

    IP experiment of HeLa using 22335-1-AP

    IP result of anti-RCC1 (IP:22335-1-AP, 3ug; Detection:22335-1-AP 1:1500) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RCC1 Polyclonal antibody (22335-1-AP)

    IHC staining of mouse testis using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RCC1 Polyclonal antibody (22335-1-AP)

    IHC staining of mouse testis using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RCC1 Polyclonal antibody (22335-1-AP)

    IHC staining of human small intestine using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RCC1 Polyclonal antibody (22335-1-AP)

    IHC staining of human small intestine using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 22335-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RCC1 Polyclonal antibody (22335-1-AP)

    IHC staining of human testis using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RCC1 Polyclonal antibody (22335-1-AP)

    IHC staining of human testis using 22335-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22335-1-AP (RCC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RCC1 Polyclonal antibody (22335-1-AP)

    IF Staining of HepG2 using 22335-1-AP

    Immunofluorescent analysis of HepG2 cells using 22335-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells, HepG2 cells, mouse colon tissue, rat colon tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 22335-1-AP is RCC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCC1 fusion protein Ag17809
Full Name regulator of chromosome condensation 1
Calculated Molecular Weight 421 aa, 45 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_10896498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against full length RCC1 of human origin.

Protocols

Product Specific Protocols
WB protocol for RCC1 antibody 22335-1-APDownload protocol
IHC protocol for RCC1 antibody 22335-1-APDownload protocol
IF protocol for RCC1 antibody 22335-1-APDownload protocol
IP protocol for RCC1 antibody 22335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Pathol Oncol Res

RCC1 Expression as a Prognostic Marker in Colorectal Liver Oligometastases.

Authors - Yuxiang Deng
View Article