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  • Featured Product
  • KD/KO Validated

RNASEH2A Polyclonal antibody

RNASEH2A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16132-1-AP

Synonyms

AGS4, JUNB, Ribonuclease H2 subunit A, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, RNase H(35), RNase H2 subunit A, RNase HI large subunit, RNASEH2A, RNASEHI, RNHIA, RNHL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH2A Polyclonal antibody (16132-1-AP)

    WB analysis using 16132-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using RNASEH2A Polyclonal antibody (16132-1-AP)

    WB analysis of human liver using 16132-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16132-1-AP targets RNASEH2A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASEH2A fusion protein Ag9118
Full Name ribonuclease H2, subunit A
Calculated Molecular Weight 299 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC011748
Gene Symbol RNASEH2A
Gene ID (NCBI) 10535
RRIDAB_2269729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

Protocols

Product Specific Protocols
WB protocol for RNASEH2A antibody 16132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells

Authors - Sho Sugawara
View Article
humanWB

PLoS Genet

Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.

Authors - Sarah K Deasy
  • KD Validated
View Article

Oncol Rep

RNaseH2A is involved in human gliomagenesis through the regulation of cell proliferation and apoptosis.

Authors - Bin Dai
View Article
humanIHC

Tumori

Hypoxia-induced RNASEH2A limits activation of cGAS-STING signaling in HCC and predicts poor prognosis.

Authors - Fengbo Zhao
View Article
human,mouseWB

bioRxiv

RTF2 controls replication repriming and ribonucleotide excision at the replisome

Authors - Brooke A Conti
View Article