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  • KD/KO Validated

RSK2 Polyclonal antibody

RSK2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23762-1-AP

Synonyms

CLS, HU 3, ISPK 1, ISPK1, MAPKAP kinase 1b, MAPKAPK 1b, MAPKAPK1B, MRX19, p90 RSK 3, p90 RSK2, p90RSK3, pp90RSK2, Ribosomal S6 kinase 2, RPS6KA3, RSK, RSK 2, RSK2, S6K alpha 3, S6K alpha3



Tested Applications

Positive WB detected inNIH/3T3 cells, K-562 cells, MCF-7 cells, HepG2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

The immunogen of 23762-1-AP is RSK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSK2 fusion protein Ag20620
Full Name ribosomal protein S6 kinase, 90kDa, polypeptide 3
Calculated molecular weight 740 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC096301
Gene symbol RPS6KA3
Gene ID (NCBI) 6197
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

Protocols

Product Specific Protocols
WB protocol for RSK2 antibody 23762-1-APDownload protocol
IHC protocol for RSK2 antibody 23762-1-APDownload protocol
IF protocol for RSK2 antibody 23762-1-APDownload protocol
IP protocol for RSK2 antibody 23762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

FLT3-ITD Activates RSK1 to Enhance Proliferation and Survival of AML Cells by Activating mTORC1 and eIF4B Cooperatively with PIM or PI3K and by Inhibiting Bad and BIM.

Authors - Daisuke Watanabe
humanWB

Prostate

MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components.

Authors - Zhengzheng Su
humanWB

Int J Biochem Cell Biol

DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo.

Authors - Ying Zhang
humanWB

Cell Death Differ

β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.

Authors - Jia Ma

Theranostics

S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer.

Authors - Yumei Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (09-04-2020)

Very clean bands of RSK2 in immunoblotting

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 for western
  • Cell Tissue Type: Human colon cancer cells