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  • KD/KO Validated

Raptor Polyclonal antibody

Raptor Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20984-1-AP

Synonyms

KIAA1303, raptor, RPTOR



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells , HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20984-1-AP targets Raptor in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name raptor
Calculated molecular weight 149 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberNM_020761
Gene symbol KIAA1303
Gene ID (NCBI) 57521
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.

Protocols

Product Specific Protocols
WB protocol for Raptor antibody 20984-1-APDownload protocol
IHC protocol for Raptor antibody 20984-1-APDownload protocol
IF protocol for Raptor antibody 20984-1-APDownload protocol
IP protocol for Raptor antibody 20984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Cell

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Authors - Joanna R Kovalski
human,mouseWB,IF,IHC

J Cell Physiol

Thioredoxin-interacting protein deficiency alleviates phenotypic alterations of podocytes via inhibition of mTOR activation in diabetic nephropathy.

Authors - Shan Song
  • KD Validated
humanWB

J Genet Genomics

mTOR-S6K1 pathway mediates cytoophidium assembly.

Authors - Zhe Sun
humanWB

Autophagy

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Authors - Xing Huang
WB

Biochim Biophys Acta

Ribosomal protein S6 kinase1 coordinates with TOR-Raptor2 to regulate thylakoid membrane biosynthesis in rice.

Authors - Linxiao Sun
mouseWB

Oncotarget

Arctigenin functions as a selective agonist of estrogen receptor β to restrict mTORC1 activation and consequent Th17 differentiation.

Authors - Xin Wu