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Raptor Antibody 8 Publications

Rabbit Polyclonal| Catalog number: 20984-1-AP

Featured Product KD/KO validated

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Raptor Antibody IF HeLa cells 20984-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 20984-1-AP (Raptor antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Raptor Antibody WB HepG2 cells 20984-1-AP

WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells.

Raptor Antibody IP HeLa cells 20984-1-AP

IP Result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:1000) with HeLa cells lysate 1505ug.

Raptor Antibody WB HeLa cells 20984-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 20984-1-AP( Raptor Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Raptor Antibody IHC mouse skeletal muscle tissue 20984-1-AP

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20984-1-AP (Raptor antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Raptor Antibody IHC mouse skeletal muscle tissue 20984-1-AP

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20984-1-AP (Raptor antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, rat, mouse, rice
Positive WB detected in:
HeLa cells, MCF-7 cells, HepG2 cells , HepG2 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
mouse skeletal muscle tissue, human kidney tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
raptor
Calculated molecular weight:
149 kDa
Observed molecular weight:
130-150 kDa
GenBank accession number:
NM_020761
Gene ID (NCBI):
57521
Gene symbol
KIAA1303
Synonyms
KIAA1303, raptor, RPTOR

Background

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.


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