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Raptor Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 20984-1-AP

Featured Product KD/KO validated

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Con: 119 μg/150 μl

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Tested Applications
Applications:
ELISA, IF, IHC, IP, WB
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Images See all

20984-1-AP;HepG2 cells

WB result of Raptor antibody (20984-1-AP, 1:500) with si-control and si-Raptor transfected HepG2 cells.

20984-1-AP;HeLa cells

IP Result of anti-Raptor (IP:20984-1-AP, 4ug; Detection:20984-1-AP 1:1000) with HeLa cells lysate 1505ug.

20984-1-AP;human kidney tissue

Immunohistochemical of paraffin-embedded human kidney using 20984-1-AP(RPTOR antibody) at dilution of 1:50 (under 10x lens)

20984-1-AP;human kidney tissue

Immunohistochemical of paraffin-embedded human kidney using 20984-1-AP(RPTOR antibody) at dilution of 1:50 (under 40x lens)

20984-1-AP;HeLa cells

HeLa cells were subjected to SDS PAGE followed by western blot with 20984-1-AP( Raptor Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

20984-1-AP;HeLa cells

Immunofluorescent analysis of HeLa cells using 20984-1-AP(Raptor antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, rat, mouse, rice
Positive WB detected in:
HeLa cells, MCF-7 cells, HepG2 cells , HepG2 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human kidney tissue, human kidney tissue, human skeletal muscle tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
raptor
Calculated molecular weight:
149 kDa
Observed molecular weight:
130-150 kDa
GenBank accession number:
NM_020761
Gene ID (NCBI):
57521
Gene symbol
KIAA1303
Synonyms
KIAA1303, raptor, RPTOR
Background

RPTOR, also named as KIAA1303 and RAPTOR Belongs to the WD repeat RAPTOR family. It is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. The antibody is specific to RPTOR.


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