• Featured Product
  • KD/KO Validated

CNOT9 Polyclonal antibody

CNOT9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22503-1-AP

Synonyms

CNOT9, Rcd 1, RCD1, RCD1+, RQCD1



Tested Applications

Positive WB detected inmouse thymus tissue, HepG2 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22503-1-AP is CNOT9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT9 fusion protein Ag18197
Full Name RCD1 required for cell differentiation1 homolog (S. pombe)
Calculated molecular weight 299 aa, 34 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC007102
Gene symbol RQCD1
Gene ID (NCBI) 9125
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.

Protocols

Product Specific Protocols
WB protocol for CNOT9 antibody 22503-1-APDownload protocol
IHC protocol for CNOT9 antibody 22503-1-APDownload protocol
IF protocol for CNOT9 antibody 22503-1-APDownload protocol
IP protocol for CNOT9 antibody 22503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Cell Rep

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Authors - Jessica A Hicks
humanWB

Elife

Translational control of ERK signaling through miRNA/4EHP-directed silencing.

Authors - Seyed Mehdi Jafarnejad
humanWB

Nucleic Acids Res

Conservation of miRNA-mediated silencing mechanisms across 600 million years of animal evolution.

Authors - Marta Mauri
humanWB

Mol Cell

Structural and Biochemical Insights to the Role of the CCR4-NOT Complex and DDX6 ATPase in MicroRNA Repression.

Authors - Hansruedi Mathys
humanWB

Mol Cell

A DDX6-CNOT1 Complex and W-Binding Pockets in CNOT9 Reveal Direct Links between miRNA Target Recognition and Silencing.

Authors - Ying Chen
humanWB

Cell

Phase Transitions in the Assembly and Function of Human miRISC.

Authors - Jessica Sheu-Gruttadauria