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RSAD2 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 11833-1-AP

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Con: 66 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

RSAD2 Antibody WB A549 cells 11833-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 11833-1-AP(RSAD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

RSAD2 Antibody IHC human skin tissue 11833-1-AP

Immunohistochemical of paraffin-embedded human skin using 11833-1-AP(RSAD2 antibody) at dilution of 1:50 (under 10x lens)

RSAD2 Antibody IHC human skin tissue 11833-1-AP

Immunohistochemical of paraffin-embedded human skin using 11833-1-AP(RSAD2 antibody) at dilution of 1:50 (under 40x lens)

RSAD2 Antibody IF Hela cells 11833-1-AP

Immunofluorescent analysis of Hela cells, using RSAD2 antibody 11833-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Species specificity:
human, mouse
Positive WB detected in:
A549 cells, Raji cells, mouse lung tissue
Positive IHC detected in:
human skin tissue, human osteosarcoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RSAD2 fusion protein Ag2409
Full name:
radical S-adenosyl methionine domain containing 2
Calculated molecular weight:
361aa,42 kDa
Observed molecular weight:
43-48 kDa
GenBank accession number:
BC017969
Gene ID (NCBI):
91543
Gene symbol
RSAD2
Synonyms
2510004L01Rik, cig33, cig5, RSAD2, vig1, Viperin

Background

RSAD2 (radical S-adenosyl methionine domain-containing protein 2), also known as CIG5 (cytomegalovirus-induced gene 5 protein), vig1, viperin or CIG33, displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses. Expression of the protein can be induced by interferon. Western blot analysis of IFNG-treated cells revealed 43 kD/35kd bands.


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