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  • KD/KO Validated

RSPO2 Antibody

RSPO2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17781-1-AP

Synonyms

CRISTIN2, hRspo2, R spondin 2, Roof plate specific spondin 2, RSPO2



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17781-1-AP targets RSPO2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSPO2 fusion protein Ag11216
Full Name R-spondin 2 homolog (Xenopus laevis)
Calculated molecular weight 243aa,28 kDa
Observed molecular weight 33 kDa
GenBank accession number BC027938
Gene symbol RSPO2
Gene ID (NCBI) 340419
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RSPO2 is one of the R-spondins (RSPOs) which comprise a family of four secreted proteins that have important roles in cell proliferation, cell fate determination and organogenesis. RSPOs typically exert their effects by potentiating the Wnt/beta-catenin signaling pathway. Overexpression of Rspo2 is sufficient to promote bone morphogenetic protein-induced mineralization Rspo2 was identified as a novel common integration site (CIS) for the mouse mammary tumor virus (MMTV) in viral induced mouse mammary tumors.

Protocols

Product Specific Protocols
WB protocol for RSPO2 antibody 17781-1-APDownload protocol
IHC protocol for RSPO2 antibody 17781-1-APDownload protocol
IP protocol for RSPO2 antibody 17781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

BMC Res Notes

R-spondin 2 mediates neutrophil egress into the alveolar space through increased lung permeability.

Authors - S R Jackson
  • KO Validated
humanWB

Mol Biol Rep

RSPO fusion transcripts in colorectal cancer in Japanese population.

Authors - Kazuya Shinmura
humanWB

DNA Cell Biol

Expression and Prognostic Values of the Roof Plate-Specific Spondin Family in Bladder Cancer.

Authors - Lei Gao