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RSPO3 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 17193-1-AP

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Con: 28 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

RSPO3 Antibody WB mouse brain tissue 17193-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 17193-1-AP( RSPO3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

RSPO3 Antibody IP human placenta tissue 17193-1-AP

IP Result of anti-RSPO3 (IP:17193-1-AP, 4ug; Detection:17193-1-AP 1:300) with human placenta tissue lysate 2800ug.

RSPO3 Antibody IHC human lung cancer tissue 17193-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 17193-1-AP( RSPO3 Antibody) at dilution of 1:200 (under 10x lens).

RSPO3 Antibody IHC human lung cancer tissue 17193-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 17193-1-AP( RSPO3 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, A549 cells, human placenta tissue, Transfected HEK-293 cells
Positive IP detected in:
human placenta tissue
Positive IHC detected in:
human lung cancer tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:100-1:400

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RSPO3 fusion protein Ag9899
Full name:
R-spondin 3 homolog (Xenopus laevis)
Calculated molecular weight:
272aa,31 kDa
Observed molecular weight:
40 kDa
GenBank accession number:
BC022367
Gene ID (NCBI):
84870
Gene symbol
RSPO3
Synonyms
CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2

Background

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1–4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4–6), constitute a ligand–receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.


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