- Featured Product
- KD/KO Validated
RSPO3 Polyclonal antibody
RSPO3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17193-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse small intestine tissue, Transfected HEK-293 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human lung cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
17193-1-AP targets RSPO3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RSPO3 fusion protein Ag9899 |
| Full Name | R-spondin 3 homolog (Xenopus laevis) |
| Calculated Molecular Weight | 272 aa, 31 kDa |
| Observed Molecular Weight | 30-40 kDa |
| GenBank Accession Number | BC022367 |
| Gene Symbol | RSPO3 |
| Gene ID (NCBI) | 84870 |
| RRID | AB_2878360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPO3 antibody 17193-1-AP | Download protocol |
| IHC protocol for RSPO3 antibody 17193-1-AP | Download protocol |
| IP protocol for RSPO3 antibody 17193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine. | ||
Cell Death Dis Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury
| ||
Am J Physiol Cell Physiol R-Spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells. | ||
J Exp Clin Cancer Res Subtypes in pancreatic ductal adenocarcinoma based on niche factor dependency show distinct drug treatment responses. |
