RSPO3 Polyclonal antibody

RSPO3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17193-1-AP

Synonyms

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2



Tested Applications

Positive WB detected inmouse brain tissue, A549 cells, human placenta tissue, Transfected HEK-293 cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17193-1-AP is RSPO3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSPO3 fusion protein Ag9899
Full Name R-spondin 3 homolog (Xenopus laevis)
Calculated molecular weight 272 aa, 31 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC022367
Gene symbol RSPO3
Gene ID (NCBI) 84870
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

Protocols

Product Specific Protocols
WB protocol for RSPO3 antibody 17193-1-APDownload protocol
IHC protocol for RSPO3 antibody 17193-1-APDownload protocol
IP protocol for RSPO3 antibody 17193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Physiol Cell Physiol

R-Spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells.

Authors - Zhilong Chen
humanWB

Proc Natl Acad Sci U S A

RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.

Authors - Kendra S Carmon
mouseWB

Nat Commun

R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.

Authors - Teng Han