|Positive WB detected in||mouse brain tissue, A549 cells, human placenta tissue, Transfected HEK-293 cells|
|Positive IP detected in||human placenta tissue|
|Positive IHC detected in||human lung cancer tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17193-1-AP is RSPO3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RSPO3 fusion protein Ag9899|
|Full Name||R-spondin 3 homolog (Xenopus laevis)|
|Calculated molecular weight||272 aa, 31 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC022367|
|Gene ID (NCBI)||84870|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.
Am J Physiol Cell Physiol
R-Spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells.
Proc Natl Acad Sci U S A
RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.
R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.