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- KD/KO Validated
RUVBL1 Polyclonal antibody
RUVBL1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CHIP, ELISA
Conjugate
Unconjugated
Cat no : 10210-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, rat testis tissue, HT-1080 cells, mouse heart tissue, K-562 cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse liver tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 9 publications below |
WB | See 28 publications below |
IHC | See 4 publications below |
IF | See 2 publications below |
IP | See 2 publications below |
ChIP | See 1 publications below |
Product Information
10210-2-AP targets RUVBL1 in WB, IP, IHC, IF, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RUVBL1 fusion protein Ag0281 |
Full Name | RuvB-like 1 (E. coli) |
Calculated molecular weight | 456 aa, 50 kDa |
Observed molecular weight | 50 kDa |
GenBank accession number | BC002993 |
Gene symbol | RUVBL1 |
Gene ID (NCBI) | 8607 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.
Protocols
Product Specific Protocols | |
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WB protocol for RUVBL1 antibody 10210-2-AP | Download protocol |
IHC protocol for RUVBL1 antibody 10210-2-AP | Download protocol |
IP protocol for RUVBL1 antibody 10210-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Carcinogenesis Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer. | ||
Biochem Biophys Res Commun RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis.
| ||
Cell Biochem Funct Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6. | ||
Biochim Biophys Acta RUVBL1-ITFG1 interaction is required for collective invasion in breast cancer.
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