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RUVBL1 Antibody 27 Publications

Rabbit Polyclonal| Catalog number: 10210-2-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, CHIP, ELISA
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Images See all

RUVBL1 Antibody WB HEK-293 cells 10210-2-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10210-2-AP(RUVBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

RUVBL1 Antibody IP HeLa cells 10210-2-AP

IP Result of anti-RUVBL1 (IP:10210-2-AP, 3ug; Detection:10210-2-AP 1:1000) with HeLa cells lysate 800ug.

RUVBL1 Antibody IHC mouse liver tissue 10210-2-AP

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP( RUVBL1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, HeLa cells, rat testis tissue, HT-1080 cells, mouse heart tissue, K-562 cells, NIH/3T3 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
mouse liver tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RUVBL1 fusion protein Ag0281
Full name:
RuvB-like 1 (E. coli)
Calculated molecular weight:
456aa, 50 kDa
Observed molecular weight:
50 kDa
GenBank accession number:
BC002993
Gene ID (NCBI):
8607
Gene symbol
RUVBL1
Synonyms
49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A

Background

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.


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