- Featured Product
- KD/KO Validated
RUVBL1 Polyclonal antibody
RUVBL1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CHIP, ELISA
Conjugate
Unconjugated
Cat no : 10210-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, rat testis tissue, HT-1080 cells, mouse heart tissue, K-562 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse liver tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 25 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| IP | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
10210-2-AP targets RUVBL1 in WB, IP, IHC, IF, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RUVBL1 fusion protein Ag0281 |
| Full Name | RuvB-like 1 (E. coli) |
| Calculated molecular weight | 456 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC002993 |
| Gene symbol | RUVBL1 |
| Gene ID (NCBI) | 8607 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RUVBL1 antibody 10210-2-AP | Download protocol |
| IHC protocol for RUVBL1 antibody 10210-2-AP | Download protocol |
| IP protocol for RUVBL1 antibody 10210-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Carcinogenesis Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer. | ||
Biochem Biophys Res Commun RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis.
| ||
Cell Biochem Funct Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6. | ||
Biochim Biophys Acta RUVBL1-ITFG1 interaction is required for collective invasion in breast cancer.
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