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  • KD/KO Validated

RUVBL1 Polyclonal antibody

RUVBL1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CHIP, ELISA

Conjugate

Unconjugated

Cat no : 10210-2-AP

Synonyms

49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, rat testis tissue, HT-1080 cells, mouse heart tissue, K-562 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10210-2-AP targets RUVBL1 in WB, IP, IHC, IF, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag0281
Full Name RuvB-like 1 (E. coli)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002993
Gene symbol RUVBL1
Gene ID (NCBI) 8607
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.

Protocols

Product Specific Protocols
WB protocol for RUVBL1 antibody 10210-2-APDownload protocol
IHC protocol for RUVBL1 antibody 10210-2-APDownload protocol
IP protocol for RUVBL1 antibody 10210-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Carcinogenesis

Functional genetic variants of RUVBL1 predict overall survival of Chinese patients with epithelial ovarian cancer.

Authors - Haoran Li
humanIP

Genes Dev

SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA.

Authors - Ailone Tichon
humanWB,IP,IHC

Biochem Biophys Res Commun

RUVBL1, a novel C-RAF-binding protein, activates the RAF/MEK/ERK pathway to promote lung cancer tumorigenesis.

Authors - Hui Guo
  • KO Validated
humanWB

Biochim Biophys Acta

Revealing A-Raf functions through its interactome.

Authors - Xin-Yu Zhang
humanWB

Cell Biochem Funct

Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6.

Authors - Anne-Aurélie Raymond
humanWB

Biochim Biophys Acta

RUVBL1-ITFG1 interaction is required for collective invasion in breast cancer.

Authors - Wenjun Fan
  • KD Validated