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  • KD/KO Validated

S100A8 Polyclonal antibody

S100A8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15792-1-AP

Synonyms

60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A



Tested Applications

Positive WB detected inTHP-1 cells, A549 cells, COLO 320 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

15792-1-AP targets S100A8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A8 fusion protein Ag8500
Full Name S100 calcium binding protein A8
Calculated molecular weight 93 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC005928
Gene symbol S100A8
Gene ID (NCBI) 6279
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9(16216873).

Protocols

Product Specific Protocols
WB protocol for S100A8 antibody 15792-1-APDownload protocol
IHC protocol for S100A8 antibody 15792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell

KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer.

Authors - Wenting Liao
mouseWB

Cell Death Dis

Inhibition of keratinocyte necroptosis mediated by RIPK1/RIPK3/MLKL provides a protective effect against psoriatic inflammation.

Authors - Xiaoru Duan
humanWB

Oncol Lett

PMID: 31897118

Authors - Liu Yang
mouseWB

Mol Reprod Dev

Protein profiling the differences between diabetic and normal mouse cumulus cells.

Authors - Guang-Jian Jiang
humanWB,IF

Oncotarget

PMID: 28423597

Authors - YanYang Wang
  • KD Validated
humanWB,IF

Front Cell Dev Biol

BRG1 Stimulates Endothelial Derived Alarmin MRP8 to Promote Macrophage Infiltration in an Animal Model of Cardiac Hypertrophy.

Authors - Zilong Li