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SAA2 Polyclonal antibody

SAA2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13192-1-AP

Synonyms

SAA, SAA1, SAA1/2, SAA2, Serum amyloid A protein, serum amyloid A2

Validation Data Gallery

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  • Western Blot (WB) analysis of serum from mouse injected with bacteria using SAA2 Polyclonal antibody (13192-1-AP)

    WB analysis of serum from mouse injected with bacteria using 13192-1-AP

    serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 13192-1-AP (SAA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SAA2 Polyclonal antibody (13192-1-AP)

    IHC staining of mouse liver using 13192-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13192-1-AP (SAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SAA2 Polyclonal antibody (13192-1-AP)

    IHC staining of mouse liver using 13192-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13192-1-AP (SAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SAA2 Polyclonal antibody (13192-1-AP)

    IF Staining of A549 using 13192-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SAA antibody (13192-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inserum from mouse injected with bacteria tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13192-1-AP targets SAA2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAA2 fusion protein Ag3869
Full Name serum amyloid A2
Calculated Molecular Weight 122 aa, 14 kDa
Observed Molecular Weight 12-13 kDa
GenBank Accession NumberBC020795
Gene Symbol SAA2
Gene ID (NCBI) 6289
RRIDAB_2877923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SAA2 antibody 13192-1-APDownload protocol
IHC protocol for SAA2 antibody 13192-1-APDownload protocol
IF protocol for SAA2 antibody 13192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Inflammation

Interleukin-1β Induces Intracellular Serum Amyloid A1 Expression in Human Coronary Artery Endothelial Cells and Promotes its Intercellular Exchange.

Authors - Tadeja Kuret
View Article
mouseWB

Int Immunopharmacol

Artesunate attenuates serum amyloid A-induced M1 macrophage differentiation through the promotion of PHGDH

Authors - Xinhui Lu
View Article