MEFV Polyclonal antibody

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• MEFV Polyclonal antibody (24280-1-AP)

MEFV Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 24280-1-AP Copied!

Publications (16)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Pyrin

• 

  WB analysis using 24280-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of THP-1 using 24280-1-AP

  IP result of anti-MEFV (IP:24280-1-AP, 4ug; Detection:24280-1-AP 1:5000) with THP-1 cells lysate 1820 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 24280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MEFV antibody (24280-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A431 using 24280-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MEFV (24280-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IP detected in: THP-1 cells
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24280-1-AP targets MEFV in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18000

  Product name: Recombinant human MEFV protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC101511

  Sequence: MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVQLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSP

• Full Name: Mediterranean fever
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC101511
• Gene Symbol: MEFV
• Gene ID (NCBI): 4210
• RRID: AB_2879468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15553
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.

Publications

What published studies show

The anti-Pyrin antibody (Cat# 24280-1-AP) has 16 total citations across 13 journals, including Nature, Autophagy, Science Immunology, PLoS Biology, Biomaterials, Acta Pharmacologica Sinica, Journal of Agricultural and Food Chemistry, Scientific Reports, Neurochemistry Research, and Pharmacological Research. Research fields span inflammasome activation, PANoptosis, pyroptosis, ferroptosis, cerebral ischemia-reperfusion injury, Alzheimer's disease, sepsis-induced cardiomyopathy, melanoma, and kidney injury.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nature; FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation; Authors - Caroline Junqueira
mouse	WB,IP	Autophagy; Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes; Authors - Nivedita Hegdekar
		Sci Immunol; An N-terminal CDC42 T43I variant reveals the mechanism of pyrin inflammasome activation.; Authors - Mariko Aoki
		Sci Immunol; A genotype-first approach reveals the molecular basis of pyrin inflammasome activation.; Authors - Naoya Iwata
mouse	WB	Biomaterials; Dual-targeting nuclear and mitochondrial DNA damage drives immunogenic activation via PANoptosis for synergistic magneto-thermodynamic-chemotherapy.; Authors - Lingcheng Wu
		Acta Pharmacol Sin; PYRIN m(6)A modification drives cardiomyocyte PANoptosis in sepsis-induced cardiomyopathy.; Authors - He Sun