MEFV Polyclonal antibody

MEFV Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 24280-1-AP

Synonyms

Pyrin

---

Validation Data Gallery

• 

  WB analysis of human plasma using 24280-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24280-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 24280-1-AP

  IP result of anti-MEFV (IP:24280-1-AP, 4ug; Detection:24280-1-AP 1:5000) with THP-1 cells lysate 1820 ug.

• 

  IF Staining of A431 using 24280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MEFV antibody (24280-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of A431 using 24280-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MEFV (24280-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: THP-1 cells, human plasma
• Positive IP detected in: THP-1 cells
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

24280-1-AP targets MEFV in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEFV fusion protein Ag18000
• Full Name: Mediterranean fever
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC101511
• Gene Symbol: MEFV
• Gene ID (NCBI): 4210
• RRID: AB_2879468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.

Publications

Species	Application	Title
mouse	WB,IP	Autophagy; Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes; Authors - Nivedita Hegdekar
rat	WB,IF	Neurochem Res; Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway; Authors - Hao Fang
rat	IF	Exp Ther Med; Function and mechanism of pyrin and IL-10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock.; Authors - Xin Jin
human	FC	PLoS Biol; Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever; Authors - Matthew S J Mangan
		Nature; FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation; Authors - Caroline Junqueira
mouse	IF	J Agric Food Chem; Lycopene Protects against Atrazine-Induced Kidney STING-Dependent PANoptosis through Stabilizing mtDNA via Interaction with Sam50/PHB1; Authors - Bao-Jin Yi