MEFV Polyclonal antibody

MEFV Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24280-1-AP

Synonyms

FMF, Marenostrin, Mediterranean fever, MEF, MEFV, Pyrin, TRIM20

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Validation Data Gallery

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  WB analysis of human plasma using 24280-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human plasma tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below
• FC: See 1 publications below

Product Information

The immunogen of 24280-1-AP is MEFV Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEFV fusion protein Ag18000
• Full Name: Mediterranean fever
• Calculated molecular weight: 781 aa, 86 kDa
• Observed molecular weight: 86 kDa
• GenBank accession number: BC101511
• Gene symbol: MEFV
• Gene ID (NCBI): 4210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.

Publications

Species	Application	Title
rat	IF	Exp Ther Med; Function and mechanism of pyrin and IL-10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock.; Authors - Xin Jin
human	FC	PLoS Biol; Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever; Authors - Matthew S J Mangan