MEFV Polyclonal antibody

MEFV Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 24280-1-AP

Synonyms

Pyrin



Tested Applications

Positive WB detected inTHP-1 cells, human plasma
Positive IP detected inTHP-1 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24280-1-AP targets MEFV in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEFV fusion protein Ag18000
Full Name Mediterranean fever
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC101511
Gene Symbol MEFV
Gene ID (NCBI) 4210
RRIDAB_2879468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.

Protocols

Product Specific Protocols
WB protocol for MEFV antibody 24280-1-APDownload protocol
IF protocol for MEFV antibody 24280-1-APDownload protocol
IP protocol for MEFV antibody 24280-1-APDownload protocol
FC protocol for MEFV antibody 24280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Autophagy

Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes

Authors - Nivedita Hegdekar
ratIF

Exp Ther Med

Function and mechanism of pyrin and IL-10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock.

Authors - Xin Jin
humanFC

PLoS Biol

Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever

Authors - Matthew S J Mangan

Nature

FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation

Authors - Caroline Junqueira
mouseIF

J Agric Food Chem

Lycopene Protects against Atrazine-Induced Kidney STING-Dependent PANoptosis through Stabilizing mtDNA via Interaction with Sam50/PHB1

Authors - Bao-Jin Yi