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TNF Alpha Monoclonal antibody

TNF Alpha Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat and More (5)

Applications

WB, RIP, IHC, IF, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

7B8A11

Cat no : 60291-1-Ig

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor



Tested Applications

Positive WB detected inTHP-1 cells, HeLa cells, HL-60 cells, U-937 cells, PC-12 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60291-1-Ig targets TNF Alpha in WB, RIP, IHC, IF, Cell treatment, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, pig, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TNF Alpha fusion protein Ag11413
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 17-22 kDa
GenBank accession numberBC028148
Gene symbol TNF
Gene ID (NCBI) 7124
RRIDAB_2833255
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

Protocols

Product Specific Protocols
WB protocol for TNF Alpha antibody 60291-1-IgDownload protocol
IHC protocol for TNF Alpha antibody 60291-1-IgDownload protocol
IF protocol for TNF Alpha antibody 60291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Signal Transduct Target Ther

Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms

Authors - Na Jia
humanIHC

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
humanWB

Blood

The hepatocyte-specific HNF4α/miR-122 pathway contributes to iron overload-mediated hepatic inflammation.

Authors - Min Li
human,ratWB

ACS Nano

Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis

Authors - Xia Niu
mouse,ratWB

Nat Commun

Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy

Authors - Xiaoyu Liu
mouseWB

Brain Behav Immun

Oxytocin alleviates cognitive and memory impairments by decreasing hippocampal microglial activation and synaptic defects via OXTR/ERK/STAT3 pathway in a mouse model of sepsis-associated encephalopathy

Authors - Junliang Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (04-19-2023)

Worked well for WB in U2OS cells at 1:1000. TNF-alpha observed in upper membrane part

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS
TNF Alpha Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:60291-1-Ig)
FH

Feng-Qian (Verified Customer) (12-07-2018)

Signal was very weak.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1/250
  • Cell Tissue Type: HaCaT