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  • KD/KO Validated

TNF Alpha Monoclonal antibody

TNF Alpha Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat and More (4)

Applications

WB, RIP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

7B8A11

Cat no : 60291-1-Ig

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-a, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor



Tested Applications

Positive WB detected inU-937 cells, THP-1 cells, HeLa cells, HL-60 cells, 4T1 cells, PC-12 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60291-1-Ig targets TNF Alpha in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, chicken, monkey, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TNF Alpha fusion protein Ag11413
Full Name tumor necrosis factor (TNF superfamily, member 2)
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 17-22 kDa
GenBank accession numberBC028148
Gene symbol TNF
Gene ID (NCBI) 7124
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.

Protocols

Product Specific Protocols
WB protocol for TNF Alpha antibody 60291-1-IgDownload protocol
IHC protocol for TNF Alpha antibody 60291-1-IgDownload protocol
IF protocol for TNF Alpha antibody 60291-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
humanWB

Blood

The hepatocyte-specific HNF4α/miR-122 pathway contributes to iron overload-mediated hepatic inflammation.

Authors - Min Li
mouseWB

Int J Biol Sci

Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury

Authors - Juan Zhang
humanWB

Biomaterials

Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.

Authors - Shaojun Pan
mouseWB

Biomaterials

Reactive oxygen species-responsive dexamethasone-loaded nanoparticles for targeted treatment of rheumatoid arthritis via suppressing the iRhom2/TNF-α/BAFF signaling pathway.

Authors - Rongrong Ni
humanIHC

Clin Cancer Res

Upregulation of C/EBPα Inhibits Suppressive Activity of Myeloid Cells and Potentiates Antitumor Response in Mice and Patients with Cancer.

Authors - Ayumi Hashimoto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (04-19-2023)

Worked well for WB in U2OS cells at 1:1000. TNF-alpha observed in upper membrane part

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS
TNF Alpha Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:60291-1-Ig)
FH

Feng-Qian (Verified Customer) (12-07-2018)

Signal was very weak.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1/250
  • Cell Tissue Type: HaCaT