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- KD/KO Validated
TNF Alpha Monoclonal antibody
TNF Alpha Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
human, mouse, rat and More (4)
WB, RIP, IHC, IF, ELISA
Cat no : 60291-1-Ig
Validation Data Gallery
|Positive WB detected in||U-937 cells, THP-1 cells, HeLa cells, HL-60 cells, 4T1 cells, PC-12 cells, HSC-T6 cells|
|Positive IHC detected in||human breast cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 219 publications below|
|IHC||See 64 publications below|
|IF||See 26 publications below|
|ELISA||See 3 publications below|
|RIP||See 1 publications below|
60291-1-Ig targets TNF Alpha in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, chicken, monkey, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||TNF Alpha fusion protein Ag11413|
|Full Name||tumor necrosis factor (TNF superfamily, member 2)|
|Calculated molecular weight||233 aa, 26 kDa|
|Observed molecular weight||17-22 kDa|
|GenBank accession number||BC028148|
|Gene ID (NCBI)||7124|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.
|Product Specific Protocols|
|WB protocol for TNF Alpha antibody 60291-1-Ig||Download protocol|
|IHC protocol for TNF Alpha antibody 60291-1-Ig||Download protocol|
|IF protocol for TNF Alpha antibody 60291-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (04-19-2023)
Worked well for WB in U2OS cells at 1:1000. TNF-alpha observed in upper membrane part
Feng-Qian (Verified Customer) (12-07-2018)
Signal was very weak.