|Positive WB detected in||PC-12 cells, HeLa cells, U-937 cells, LPS treated RAW 264.7 cells, 4T1 cells, RAW 264.7 cells, HSC-T6 cells, HL-60 cells|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60291-1-Ig targets TNF Alpha in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||chicken, human, monkey|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||TNF Alpha fusion protein Ag11413|
|Full Name||tumor necrosis factor (TNF superfamily, member 2)|
|Calculated molecular weight||233 aa, 26 kDa|
|Observed molecular weight||17-22 kDa|
|GenBank accession number||BC028148|
|Gene ID (NCBI)||7124|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Feng-Qian (Verified Customer) (12-07-2018)
Signal was very weak.