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  • KD/KO Validated

SCNN1A Antibody

SCNN1A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 10924-2-AP

Synonyms

Alpha ENaC, Alpha NaCH, ENaCa, ENaCalpha, SCNEA, SCNN1, SCNN1A



Tested Applications

Positive WB detected inPC-13 cells, HEK-293
Positive IHC detected inhuman kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10924-2-AP targets SCNN1A in IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCNN1A fusion protein Ag1325
Full Name sodium channel, nonvoltage-gated 1 alpha
Calculated molecular weight 75.7 kDa
Observed molecular weight 60-70 kDa
GenBank accession number BC006526
Gene symbol SCNN1A
Gene ID (NCBI) 6337
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

SCNN1A (sodium channel, non-voltage-gated 1 alpha), also known as ENaCA (epithelial Na(+) channel subunit alpha) or amiloride-sensitive sodium channel subunit alpha, is the alpha subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. ENaC consists of three different subunits: alpha, beta, gamma. Mutations in the gene of SCNN1A have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It has been reported that full-length SCNN1A protein can be cleaved into 65- and 30-kDa fragments (PMID: 16477034; 18701608).

Protocols

Product Specific Protocols
WB protocol for SCNN1A antibody 10924-2-APDownload protocol
IHC protocol for SCNN1A antibody 10924-2-APDownload protocol
IF protocol for SCNN1A antibody 10924-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Cell Physiol

Ursodeoxycholic acid stimulates alveolar fluid clearance in LPS-induced pulmonary edema via ALX/cAMP/PI3K pathway.

Authors - Fangfang Niu
mouseWB

Exp Anim

Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathway.

Authors - Bingji Jin
humanIHC

Exp Mol Pathol

Dysregulation of the epigenome in triple-negative breast cancers: Basal-like and claudin-low breast cancers express aberrant DNA hypermethylation.

Authors - Roll J Devon JD
humanWB

J Aerosol Med Pulm Drug Deliv

Hybrid Lipid/Polymer Nanoparticles for Pulmonary Delivery of siRNA: Development and Fate Upon In Vitro Deposition on the Human Epithelial Airway Barrier.

Authors - Ivana d'Angelo
humanWB

Placenta

ENaC mediates human extravillous trophblast cell line (HTR8/SVneo) invasion by regulating levels of matrix metalloproteinase 2 (MMP2).

Authors - Y Yang
  • KD Validated
mouseWB

Exp Cell Res

Neddylation-mediated Nedd4-2 activation regulates ubiquitination modification of renal NBCe1.

Authors - Jianqiao Tu