SDHA Monoclonal antibody

SDHA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, rabbit, pig and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5E10G12

Cat no : 66588-1-Ig

Synonyms

FP, SDH2, SDHA, SDHF



Tested Applications

Positive WB detected inNIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

66588-1-Ig targets SDHA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, rabbit, pig samples.

Tested Reactivity Human, Mouse, Rat, rabbit, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SDHA fusion protein Ag7023
Full Name succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 62-73 kDa
GenBank Accession NumberBC001380
Gene Symbol SDHA
Gene ID (NCBI) 6389
RRIDAB_2881948
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for SDHA antibody 66588-1-IgDownload protocol
IHC protocol for SDHA antibody 66588-1-IgDownload protocol
IF protocol for SDHA antibody 66588-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma

Authors - Lin-Lin Zhou
humanWB

Cell Rep

Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia

Authors - Yan Tang

Sci China Life Sci

Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins.

Authors - Jian Xiao
human,mouseWB

Cell Chem Biol

Aberrant human ClpP activation disturbs mitochondrial proteome homeostasis to suppress pancreatic ductal adenocarcinoma.

Authors - Pengyu Wang
humanWB

Bioorg Chem

SAR-guided development of indole-matrine hybrids as potential anticancer agents via mitochondrial stress/cytochrome c/caspase 3 signaling pathway

Authors - Lingyu Li
mouseWB

bioRxiv

FAM210A Regulates Mitochondrial Translation and Maintains Cardiac Mitochondrial Homeostasis

Authors - Jiangbin Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Hua (Verified Customer) (07-12-2023)

Great antibody for WB. Very strong signal and clean background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Mouse liver
SDHA Antibody Western Blot validation (1:10000 dilution) in Mouse liver (Cat no:66588-1-Ig)