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  • Featured Product
  • KD/KO Validated

SESN3 Polyclonal antibody

SESN3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11431-2-AP

Synonyms

SESN3, SEST3, sestrin 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of HeLa using 11431-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of HeLa using 11431-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of HEK-293 using 11431-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of HEK-293 using 11431-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of mouse liver using 11431-2-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of mouse liver using 11431-2-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of A431 using 11431-2-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of A431 using 11431-2-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of mouse ovary using 11431-2-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of HeLa using 11431-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of A549 using 11431-2-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of HeLa using 11431-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SESN3 Polyclonal antibody (11431-2-AP)

    WB analysis of HepG2 using 11431-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, A431 cells, A549 cells, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11431-2-AP targets SESN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SESN3 fusion protein Ag1961
Full Name sestrin 3
Calculated Molecular Weight 492 aa, 57 kDa
Observed Molecular Weight 36 kDa, 51-59 kDa
GenBank Accession NumberBC017296
Gene Symbol SESN3
Gene ID (NCBI) 143686
RRIDAB_2185602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Protocols

Product Specific Protocols
WB protocol for SESN3 antibody 11431-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor.

Authors - Chia-Chen Chen
  • KD Validated
View Article
mouseIHC

Proc Natl Acad Sci U S A

FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis.

Authors - Kwang Il Lee
View Article
humanWB

Elife

Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation.

Authors - Veronique Nogueira
  • KD Validated
View Article
mouseWB

Biochim Biophys Acta Mol Basis Dis

Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner

Authors - Sujuan Liu
View Article
humanWB,IHC

Osteoarthritis Cartilage

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.

Authors - T Shen
View Article
humanWB

PLoS One

Regulatory Effects of Sestrin 3 (SESN3) in BCR-ABL Expressing Cells.

Authors - Vakana Eliza E
View Article