SH2B1 Polyclonal antibody

SH2B1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12226-1-AP

Synonyms

KIAA1299, PSM, SH2B, SH2B adapter protein 1, SH2B adaptor protein 1, SH2B1



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12226-1-AP targets SH2B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH2B1 fusion protein Ag2863
Full Name SH2B adaptor protein 1
Calculated molecular weight 426 aa, 45 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC010704
Gene symbol SH2B1
Gene ID (NCBI) 25970
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH2B1 is a member of the SH2-domain containing mediators family. SH2B1 has been shown to interact with INS receptor, Grb2, TrkA and Janus kinase 2. Variations close to or in the SH2B1 gene have been found to associate with obesity in two large genome wide association studies of body mass index (PMID: 19079260). SH2B1deletions are associated with severe early-onset obesity (PMID: 19966786).

Protocols

Product Specific Protocols
WB protocol for SH2B1 antibody 12226-1-APDownload protocol
IHC protocol for SH2B1 antibody 12226-1-APDownload protocol
IF protocol for SH2B1 antibody 12226-1-APDownload protocol
IP protocol for SH2B1 antibody 12226-1-APDownload protocol
Standard Protocols
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