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  • Featured Product
  • KD/KO Validated

SLC25A10 Polyclonal antibody

SLC25A10 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12086-1-AP

Synonyms

DIC, SLC25A10

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SLC25A10 Polyclonal antibody (12086-1-AP)

    WB analysis of mouse liver using 12086-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC25A10 Polyclonal antibody (12086-1-AP)

    WB analysis using 12086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SLC25A10 Polyclonal antibody (12086-1-AP)

    WB analysis of mouse kidney using 12086-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SLC25A10 Polyclonal antibody (12086-1-AP)

    WB analysis of A549 using 12086-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SLC25A10 Polyclonal antibody (12086-1-AP)

    IP experiment of mouse liver using 12086-1-AP

    IP result of anti-SLC25A10 (IP:12086-1-AP, 3ug; Detection:12086-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human lung tissue using SLC25A10 Polyclonal antibody (12086-1-AP)

    IHC staining of human lung using 12086-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using SLC25A10 Polyclonal antibody (12086-1-AP)

    IHC staining of human lung using 12086-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A10 Polyclonal antibody (12086-1-AP)

    IHC staining of mouse kidney using 12086-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SLC25A10 Polyclonal antibody (12086-1-AP)

    IHC staining of mouse kidney using 12086-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, PC-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman lung tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12086-1-AP targets SLC25A10 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A10 fusion protein Ag2719
Full Name solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
Calculated Molecular Weight 296 aa, 32 kDa
Observed Molecular Weight 29-31 kDa
GenBank Accession NumberBC015797
Gene Symbol SLC25A10
Gene ID (NCBI) 1468
RRIDAB_10859816
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A10, also known as dicarboxylate ion carrier (DIC), is a mitochondrial inner membrane carrier protein which catalyzes the transport of dicarboxylates such as malate and succinate across the mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate. SLC25A10 is expressed at highest levels in kidney and liver, lower levels in lung, spleen, heart, and brain, and lowest levels in skeletal muscle. Two isoforms of SLC25A10 exist due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SLC25A10 antibody 12086-1-APDownload protocol
IHC protocol for SLC25A10 antibody 12086-1-APDownload protocol
IP protocol for SLC25A10 antibody 12086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Hum Cell

The role of PYCR1 in inhibiting 5-fluorouracil-induced ferroptosis and apoptosis through SLC25A10 in colorectal cancer

Authors - Borong Zhou
View Article
humanWB,IHC

Oncol Lett

SLC25A10 performs an oncogenic role in human osteosarcoma.

Authors - Gaoyuan Wang
  • KD Validated
View Article