ANT1/2 Polyclonal antibody

ANT1/2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17796-1-AP

Synonyms

2F1, AAC2, ADP,ATP carrier protein 2, ADP/ATP translocase 2, ANT 2, ANT1/2, SLC25A5, T2, T3



Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17796-1-AP targets ANT1/2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANT1/2 fusion protein Ag11956
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
Calculated molecular weight 298 aa, 33 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC056160
Gene symbol SLC25A5
Gene ID (NCBI) 292
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimmer of ANT1/2. (22423112)

Protocols

Product Specific Protocols
WB protocol for ANT1/2 antibody 17796-1-APDownload protocol
IHC protocol for ANT1/2 antibody 17796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.

Authors - Da-Wei Liu
humanIHC

Mol Imaging Biol

Adenine Nucleotide Translocase 2 as an Enzyme Related to [18F] FDG Accumulation in Various Cancers.

Authors - Chul-Hee Lee
humanWB

J Proteome Res

Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.

Authors - Foteini Violitzi
ratWB

Exp Neurol

Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder.

Authors - Jin-Hee Seo
humanIHC

Mol Cell Proteomics

Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans.

Authors - Kathleen C Lundberg
mouseWB

PLoS Genet

Novel insights into SLC25A46-related pathologies in a genetic mouse model.

Authors - Maria Eirini Terzenidou