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GLUT3 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 20403-1-AP

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Con: 49 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

GLUT3 Antibody WB HepG2 cells 20403-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 20403-1-AP( GLUT3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

GLUT3 Antibody IHC human lung cancer tissue 20403-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20403-1-AP( GLUT3 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

GLUT3 Antibody IHC human lung cancer tissue 20403-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 20403-1-AP( GLUT3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse
Positive WB detected in:
HepG2 cells, Jurkat cells, mouse brain tissue, HeLa cells, HEK-293 cells
Positive IHC detected in:
human lung cancer tissue, human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GLUT3 fusion protein Ag14203
Full name:
solute carrier family 2 (facilitated glucose transporter), member 3
Calculated molecular weight:
496aa,54 kDa
Observed molecular weight:
48-60 kDa
GenBank accession number:
BC039196
Gene ID (NCBI):
6515
Gene symbol
SLC2A3
Synonyms
GLUT 3, GLUT3, SLC2A3

Background

Glucose transporter 3 (GLUT3), also known as solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3), is a transporter protein regulating glucose transport across cell membranes and is a primary glucose transporter in neurons.

What is the molecular weight of GLUT3? Is GLUT3 post-translationally modified?

The molecular weight of GLUT3 transporter is 49-52 kDa and depends on the glycosylation pattern, which is tissue-specific (PMID: 9253355 and 9889355). GLUT3 can be N-glycosylated.

What is the subcellular localization of GLUT3?

Glucose transporters, including GLUT3, are multiple-pass integral membrane proteins. GLUT3 is present at the plasma membrane but is also a subject of recycling between the plasma membrane and endosomes. 

What molecules can be transported by GLUT3?

Although the main substrate of GLUT3 transport is glucose, it can also transport mannose, galactose, and xylose.

What is the tissue expression pattern of GLUT3?

GLUT1 and GLUT3 are important for the transport of glucose into the central nervous system. While GLUT1 transports glucose through blood vessels and into astrocytes, GLUT3 is predominantly expressed in neurons, being their main glucose transporter (PMID: 9302083), and in testis (PMID: 18577699).



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