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SLC39A9 Polyclonal antibody

SLC39A9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17607-1-AP

Synonyms

SLC39A9, Zinc transporter ZIP9, ZIP 9, ZIP9, Zrt and Irt like protein 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC39A9 Polyclonal antibody (17607-1-AP)

    WB analysis using 17607-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17607-1-AP (SLC39A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC39A9 Polyclonal antibody (17607-1-AP)

    IHC staining of human placenta using 17607-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17607-1-AP (SLC39A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SLC39A9 Polyclonal antibody (17607-1-AP)

    IHC staining of human placenta using 17607-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17607-1-AP (SLC39A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHUVEC cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17607-1-AP targets SLC39A9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC39A9 fusion protein Ag11752
Full Name solute carrier family 39 (zinc transporter), member 9
Calculated Molecular Weight 307 aa, 32 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC047682
Gene Symbol SLC39A9
Gene ID (NCBI) 55334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc transporter ZIP9 subfamily (SLC39A9) encodes a protein with characteristics of a membrane androgen receptor (mAR). Human ZIP9 is a 307-amino acid protein, which has eight putative transmembrane domains. ZIP9 has also been identified and characterized as a resident protein in the Golgi in DT40 and HeLa cell lines (PMID: 19420709, 23505453). ZIP9 may be present on the cell surface, as well as nuclear and mitochondrial membranes, in human breast and prostate cancer cell lines, where it mediates testosterone induction of apoptosis (PMID: 29952128). knockout of ZIP9 would lead to zinc accumulation in the secretory pathway, which could influence the general ion homeostasis and in turn the glycosylation outcome (PMID: 36648436).

Protocols

Product Specific Protocols
WB protocol for SLC39A9 antibody 17607-1-APDownload protocol
IHC protocol for SLC39A9 antibody 17607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols