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WB analysis using 15193-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 15193-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC staining of rat testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15193-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 15193-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

CD98/SLC3A2 Polyclonal antibody

Name: CD98/SLC3A2 Polyclonal antibody
Brand: Proteintech
SKU: 15193-1-AP

CD98/SLC3A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15193-1-AP

Publications(60)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CD98, SLC3A2, 4F2 cell-surface antigen heavy chain, Amino acid transporter heavy chain SLC3A2, MDU1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (60)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, Raji cells, HepG2 cells
Positive IHC detected in	rat testis tissue, mouse testis tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 49 publications below
IHC	See 10 publications below
IF	See 13 publications below
IP	See 1 publications below

Product Information

15193-1-AP targets CD98/SLC3A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7280 Product name: Recombinant human SLC3A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 269-630 aa of BC001061 Sequence: KDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA Predict reactive species
Full Name	solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated Molecular Weight	68 kDa
Observed Molecular Weight	85-94 kDa, 120-130 kDa
GenBank Accession Number	BC001061
Gene Symbol	CD98
Gene ID (NCBI)	6520
RRID	AB_2254909
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08195
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Protocols

Product Specific Protocols
IF protocol for CD98/SLC3A2 antibody 15193-1-AP	Download protocol
IHC protocol for CD98/SLC3A2 antibody 15193-1-AP	Download protocol
WB protocol for CD98/SLC3A2 antibody 15193-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	ACS Nano Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy Authors - Rangrang Fan View Article
human	WB,IHC	Sci Transl Med Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain. Authors - Kana Hasegawa View Article
mouse	IF	Nat Commun Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters. Authors - Weiwei Yu View Article
human	IF	J Allergy Clin Immunol Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function. Authors - Siddhartha S Saha View Article
mouse,human	WB,IHC,IF	JACC Basic Transl Sci CircBTBD7-420aa Encoded by hsa_circ_0000563 Regulates the Progression of Atherosclerosis and Construction of circBTBD7-420aa Engineered Exosomes Authors - Xiongkang Gan View Article
human	WB	J Hazard Mater ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes. Authors - Xuan Lai View Article