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CD98/SLC3A2 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 15193-1-AP

Featured Product KD/KO validated

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Con: 52 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

CD98/SLC3A2 Antibody WB HeLa cells 15193-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15193-1-AP( CD98 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours

CD98/SLC3A2 Antibody IHC human testis tissue 15193-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 15193-1-AP( CD98 antibody at dilution of 1:5000 (under 10x lens).

CD98/SLC3A2 Antibody IHC human testis tissue 15193-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 15193-1-AP( CD98 antibody at dilution of 1:5000 (under 40x lens).

CD98/SLC3A2 Antibody IF HepG2 cells 15193-1-AP

Immunofluorescent analysis of HepG2 cells, using SLC3A2 antibody 15193-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CD98/SLC3A2 Antibody FC HepG2 cells 15193-1-AP

1X10^6 HepG2 cells were stained with .2ug CD98 antibody (15193-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
HeLa cells, Raji cells
Positive IHC detected in:
human testis tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:5000-1:20000
IHC : 1:2500-1:10000
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD98/SLC3A2 fusion protein Ag7280
Full name:
solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight:
68 kDa
Observed molecular weight:
85 kDa,120 kDa
GenBank accession number:
BC001061
Gene ID (NCBI):
6520
Gene symbol
SLC3A2
Synonyms
4F2, 4F2 heavy chain antigen, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, SLC3A2

Background

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).


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