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CD98/SLC3A2 Polyclonal antibody

CD98/SLC3A2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15193-1-AP

Synonyms

4F2, 4F2 heavy chain antigen, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, SLC3A2



Tested Applications

Positive WB detected inHeLa cells, Raji cells
Positive IHC detected inhuman testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15193-1-AP targets CD98/SLC3A2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD98/SLC3A2 fusion protein Ag7280
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight 68 kDa
Observed molecular weight 85 kDa, 120 kDa
GenBank accession numberBC001061
Gene symbol SLC3A2
Gene ID (NCBI) 6520
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Protocols

Product Specific Protocols
WB protocol for CD98/SLC3A2 antibody 15193-1-APDownload protocol
IHC protocol for CD98/SLC3A2 antibody 15193-1-APDownload protocol
IF protocol for CD98/SLC3A2 antibody 15193-1-APDownload protocol
FC protocol for CD98/SLC3A2 antibody 15193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hepatol Int

Upregulation of CD147 protects hepatocellular carcinoma cell from apoptosis through glycolytic switch via HIF-1 and MCT-4 under hypoxia.

Authors - Xia Ke
humanWB,IHC

Oncol Rep

SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway.

Authors - Bin Zhu
  • KD Validated
WB

J Dairy Sci

Prolactin regulates LAT1 expression via STAT5 (signal transducer and activator of transcription 5) signaling in mammary epithelial cells of dairy cows.

Authors - Jinyu Zhou
humanWB,IF

Int J Mol Sci

Dominant Suppression of β1 Integrin by Ectopic CD98-ICD Inhibits Hepatocellular Carcinoma Progression.

Authors - Bo Wu
humanIHC,IF,IP,WB

Ann Surg Oncol

CD147-CD98hc Complex Contributes to Poor Prognosis of Non-Small Cell Lung Cancer Patients Through Promoting Cell Proliferation Via the PI3K/Akt Signaling Pathway.

Authors - Fei Fei