|Positive WB detected in||HeLa cells, Raji cells|
|Positive IHC detected in||human testis tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15193-1-AP targets CD98/SLC3A2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD98/SLC3A2 fusion protein Ag7280|
|Full Name||solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2|
|Calculated molecular weight||68 kDa|
|Observed molecular weight||85 kDa, 120 kDa|
|GenBank accession number||BC001061|
|Gene ID (NCBI)||6520|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).
|Product Specific Protocols|
|WB protocol for CD98/SLC3A2 antibody 15193-1-AP||Download protocol|
|IHC protocol for CD98/SLC3A2 antibody 15193-1-AP||Download protocol|
|IF protocol for CD98/SLC3A2 antibody 15193-1-AP||Download protocol|
|FC protocol for CD98/SLC3A2 antibody 15193-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Upregulation of CD147 protects hepatocellular carcinoma cell from apoptosis through glycolytic switch via HIF-1 and MCT-4 under hypoxia.
SLC3A2 is upregulated in human osteosarcoma and promotes tumor growth through the PI3K/Akt signaling pathway.
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Prolactin regulates LAT1 expression via STAT5 (signal transducer and activator of transcription 5) signaling in mammary epithelial cells of dairy cows.
Int J Mol Sci
Dominant Suppression of β1 Integrin by Ectopic CD98-ICD Inhibits Hepatocellular Carcinoma Progression.
Ann Surg Oncol
CD147-CD98hc Complex Contributes to Poor Prognosis of Non-Small Cell Lung Cancer Patients Through Promoting Cell Proliferation Via the PI3K/Akt Signaling Pathway.