SMAD3 Monoclonal antibody

SMAD3 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1


Human, mouse and More (1)







Cat no : 66516-1-Ig


hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66516-1-Ig targets SMAD3 in WB,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name SMAD family member 3
Calculated molecular weight 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberNM_005902
Gene symbol SMAD3
Gene ID (NCBI) 4088
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.


Product Specific Protocols
WB protocol for SMAD3 antibody 66516-1-IgDownload protocol
Standard Protocols
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Cell Cycle

miR-96-5p regulated TGF-β/SMAD signaling pathway and suppressed endometrial cell viability and migration via targeting TGFBR1.

Authors - Silei Chen

Int Immunopharmacol

Amygdalin inhibits TGFβ1-induced activation of hepatic stellate cells (HSCs) in vitro and CCl4-induced hepatic fibrosis in rats in vivo.

Authors - Ruoyu Wang