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SMAD4 Monoclonal antibody

SMAD4 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

3H5H1

Cat no : 60182-1-Ig

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD4 Monoclonal antibody (60182-1-Ig)

    IHC staining of human breast cancer using 60182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD4 Monoclonal antibody (60182-1-Ig)

    IHC staining of human breast cancer using 60182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD4 Monoclonal antibody (60182-1-Ig)

    IHC staining of human breast cancer using 60182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SMAD4 Monoclonal antibody (60182-1-Ig)

    IHC staining of human breast cancer using 60182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SMAD4 Monoclonal antibody (60182-1-Ig)

    IHC staining of human lung cancer using 60182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SMAD4 Monoclonal antibody (60182-1-Ig)

    IHC staining of human lung cancer using 60182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 40x lens).

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60182-1-Ig targets SMAD4 in ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SMAD4 fusion protein Ag0299
Full Name SMAD family member 4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a mouse monoclonal antibody raised against an internal region of human SMAD4.

Protocols

Product Specific Protocols
IHC protocol for SMAD4 antibody 60182-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols