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SMAD7 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 25840-1-AP

Featured Product KD/KO validated

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Con: 81 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

SMAD7 Antibody WB L02 cells 25840-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 25840-1-AP (SMAD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

SMAD7 Antibody WB mouse liver tissue 25840-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 25840-1-AP (SMAD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

SMAD7 Antibody WB HepG2 cells 25840-1-AP

WB result of SMAD7 antibody (25840-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD7 transfected HepG2 cells

SMAD7 Antibody IHC human liver tissue 25840-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 25840-1-AP( SMAD7 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse liver tissue, L02 cells, HepG2 cells, mouse lung tissue, mouse skeletal muscle tissue, mouse kidney tissue
Positive IHC detected in:
human liver tissue, human hepatocirrhosis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SMAD7 fusion protein Ag13688
Full name:
SMAD family member 7
Calculated molecular weight:
426aa,46 kDa
Observed molecular weight:
46 kDa
GenBank accession number:
BC074819
Gene ID (NCBI):
4092
Gene symbol
SMAD7
Synonyms
CRCS3, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, SMAD 7, SMAD family member 7, SMAD7

Background

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.


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