Tested Applications
| Positive WB detected in | HeLa cells, A431 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human medulloblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12018-1-AP targets BAF170 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2634 Product name: Recombinant human BAF170 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 300-647 aa of BC013045 Sequence: KRSPSPSPTPEAKKKNAKKGPSTPYTKSKRGHREEEQEDLTKDMDEPSPVPNVEEVTLPKTVNTKKDSESAPVKGGTMTDLDEQEDESMETTGKDEDENSTGNKGEQTKNPDLHEDNVTEQTHHIIIPSYAAWFDYNSVHAIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNLAGDVCAIMRVHAFLEQWGLINYQVDAESRPTPMGPPPTSHFHVLADTPSGLVPLQPKTPQGRQVDADTKAGRKGKELDDLVPETAKGKPELQTSASQQMLNFPDKGKEKPTDMQNFGLRTDMYTKKNVPSKSKAAASATREWTEQETLLLLEALEMY Predict reactive species |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 |
| Calculated Molecular Weight | 1214 aa, 132 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC013045 |
| Gene Symbol | BAF170 |
| Gene ID (NCBI) | 6601 |
| RRID | AB_2192010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BAF170 antibody 12018-1-AP | Download protocol |
| IHC protocol for BAF170 antibody 12018-1-AP | Download protocol |
| IP protocol for BAF170 antibody 12018-1-AP | Download protocol |
| WB protocol for BAF170 antibody 12018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SMARCC2 antibody (Cat# 12018-1-AP) has 7 total citations published in journals including Molecular Cell, Genome Medicine, Cell & Molecular Biology Letters, Cell Mol Gastroenterol Hepatol, Mediators of Inflammation, Biochemical Biophysical Research Communications, and Aging. Research fields span transcription regulation, breast cancer, ovarian clear cell carcinoma, hepatocarcinogenesis, rheumatoid arthritis with atherosclerosis, and pancreatic cancer. Applications include WB and IP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Cell SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. | ||
Cell Mol Biol Lett A novel SWI/SNF complex promotes triple-negative breast cancer progression | ||
Genome Med Comprehensive molecular characterization identifies therapeutic vulnerabilities in ovarian clear cell carcinoma. | ||
Cell Mol Gastroenterol Hepatol The Ubiquitin E3 Ligase TRIM21 Promotes Hepatocarcinogenesis by Suppressing the p62-Keap1-Nrf2 Antioxidant Pathway. | ||
Mediators Inflamm Identification of Immune-Related Lactylation Genes in Rheumatoid Arthritis With Atherosclerosis: A Comprehensive Analysis Using Bulk and Single-Cell RNA Sequencing Data. | ||
Biochem Biophys Res Commun SMARCC2 silencing suppresses oncogenic activation through modulation of chromatin accessibility in breast cancer
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