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SNAI1 Polyclonal antibody

SNAI1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 13099-1-AP

Synonyms

Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1



Tested Applications

Positive WB detected inmouse heart tissue, COLO 320 cells, human heart tissue, PC-3 cells, BxPC-3 cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13099-1-AP targets SNAI1 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNAI1 fusion protein Ag3723
Full Name snail homolog 1 (Drosophila)
Calculated molecular weight 264 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC012910
Gene symbol SNAI1
Gene ID (NCBI) 6615
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Protocols

Product Specific Protocols
WB protocol for SNAI1 antibody 13099-1-APDownload protocol
IP protocol for SNAI1 antibody 13099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

Nuclear factor I/B promotes colorectal cancer cell proliferation, epithelial-mesenchymal transition and 5-fluorouracil resistance.

Authors - ZhengYi Liu
humanWB

Aging (Albany NY)

A CREB1/miR-433 reciprocal feedback loop modulates proliferation and metastasis in colorectal cancer.

Authors - Li Yan
humanIHC

J Cell Mol Med

Tn antigen promotes human colorectal cancer metastasis via H-Ras mediated epithelial-mesenchymal transition activation.

Authors - Zhe Liu
mouseWB

Nat Med

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Authors - Yichen Xu
humanWB

Cancer Cell Int

SIRT6/HIF-1α axis promotes papillary thyroid cancer progression by inducing epithelial-mesenchymal transition.

Authors - Zhou Yang
humanIF

Sci Rep

TGF-β-SNAIL axis induces Müller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane.

Authors - Atsuhiro Kanda