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  • KD/KO Validated

SOCS3 Polyclonal antibody

SOCS3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14025-1-AP

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SSI 3, SSI3, STAT induced STAT inhibitor 3



Tested Applications

Positive WB detected inHeLa cells, A375 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman heart tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14025-1-AP targets SOCS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOCS3 fusion protein Ag5170
Full Name suppressor of cytokine signaling 3
Calculated molecular weight 25 kDa
Observed molecular weight 22-30 kDa
GenBank accession numberBC060858
Gene symbol SOCS3
Gene ID (NCBI) 9021
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Observed MW of SOCS3 is from 22-30 kDa(PMID: 24827536,PMID: 26260587, PMID: 26797799), consistent with the result of Catalog#14025-1-AP.

Protocols

Product Specific Protocols
WB protocol for SOCS3 antibody 14025-1-APDownload protocol
IHC protocol for SOCS3 antibody 14025-1-APDownload protocol
IF protocol for SOCS3 antibody 14025-1-APDownload protocol
IP protocol for SOCS3 antibody 14025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

J Clin Invest

Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1.

Authors - Gaoxiang Zhao
  • KO Validated
mouseWB

Cell Death Dis

m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner.

Authors - Ruifan Wu
humanIHC

Oncogene

PMID: 30918330

Authors - Mingqing Wu
humanWB

Int J Mol Sci

Exosome-Mediated Delivery of Inducible miR-423-5p Enhances Resistance of MRC-5 Cells to Rabies Virus Infection.

Authors - Jingyu Wang
humanWB

Colloids Surf B Biointerfaces

Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.

Authors - Yanyan Zhu
humanWB

Toxicol Appl Pharmacol

PMID: 29031523

Authors - Orawan Wonganan