|Positive WB detected in||HeLa cells, A375 cells, K-562 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human heart tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14025-1-AP targets SOCS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||chicken, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SOCS3 fusion protein Ag5170|
|Full Name||suppressor of cytokine signaling 3|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||22-30 kDa|
|GenBank accession number||BC060858|
|Gene ID (NCBI)||9021|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Observed MW of SOCS3 is from 22-30 kDa(PMID: 24827536,PMID: 26260587, PMID: 26797799), consistent with the result of Catalog#14025-1-AP.
|Product Specific Protocols|
|WB protocol for SOCS3 antibody 14025-1-AP||Download protocol|
|IHC protocol for SOCS3 antibody 14025-1-AP||Download protocol|
|IF protocol for SOCS3 antibody 14025-1-AP||Download protocol|
|IP protocol for SOCS3 antibody 14025-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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