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SOCS3 Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 14025-1-AP

Featured Product KD/KO validated

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Con: 34 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

SOCS3 Antibody WB HeLa cells 14025-1-AP

WB result of SOCS3 antibody (14025-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOCS3 transfected HeLa cells

SOCS3 Antibody IHC human heart tissue 14025-1-AP

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14025-1-AP (SOCS3 antibody) at dilution of 1:200 (under 10x lens).

SOCS3 Antibody IHC human heart tissue 14025-1-AP

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14025-1-AP (SOCS3 antibody) at dilution of 1:200 (under 40x lens).

SOCS3 Antibody IF Hela cells 14025-1-AP

Immunofluorescent analysis of Hela cells, using SOCS3 antibody 14025-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SOCS3 Antibody IP K-562 cells 14025-1-AP

IP Result of anti-SOCS3 (IP:14025-1-AP, 4ug; Detection:14025-1-AP 1:500) with K-562 cells lysate 1720ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, HepG2 cells, K-562 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human heart tissue, human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells, HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SOCS3 fusion protein Ag5170
Full name:
suppressor of cytokine signaling 3
Calculated molecular weight:
25 kDa
Observed molecular weight:
22-30 kDa
GenBank accession number:
BC060858
Gene ID (NCBI):
9021
Gene symbol
SOCS3
Synonyms
ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SSI 3, SSI3, STAT induced STAT inhibitor 3

Background

The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Observed MW of SOCS3 is from 22-30 kDa(PMID: 24827536,PMID: 26260587, PMID: 26797799), consistent with the result of Catalog#14025-1-AP.


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