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SOX15 Polyclonal antibody

SOX15 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25415-1-AP

Synonyms

Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX15 Polyclonal antibody (25415-1-AP)

    IHC staining of mouse embryo using 25415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 25415-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX15 Polyclonal antibody (25415-1-AP)

    IHC staining of mouse embryo using 25415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 25415-1-AP (SOX15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using SOX15 Polyclonal antibody (25415-1-AP)

    IF Staining of mouse embryo using 25415-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25415-1-AP (SOX15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse embryo tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25415-1-AP targets SOX15 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOX15 fusion protein Ag22032
Full Name SRY (sex determining region Y)-box 15
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC000985
Gene Symbol SOX15
Gene ID (NCBI) 6665
RRIDAB_2880067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOX15, also named as SOX12, SOX20, is a 223 amino acid protein, which contains one HMG box DNA-binding domain. SOX15 is widely expressed in fetal and adult tissues and highest level is found in fetal spinal cord and adult brain and testis. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling. Sox15 is required for skeletal muscle regeneration and is up regulated in the embryonic mouse testis.

Protocols

Product Specific Protocols
IHC protocol for SOX15 antibody 25415-1-APDownload protocol
IF protocol for SOX15 antibody 25415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Transcription factor SOX15 regulates stem cell pluripotency and promotes neural fate during differentiation by activating the neurogenic gene Hes5

Authors - Eun-Bee Choi
  • KO Validated
View Article