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SOX15 Polyclonal antibody

SOX15 Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25415-1-AP

Synonyms

Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27

Validation Data Gallery

  • IHC staining of mouse embryo using 25415-1-AP

    IHC staining of mouse embryo using 25415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 25415-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse embryo using 25415-1-AP

    IF Staining of mouse embryo using 25415-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25415-1-AP (SOX15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse embryo tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25415-1-AP is SOX15 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOX15 fusion protein Ag22032
Full Name SRY (sex determining region Y)-box 15
Calculated molecular weight 25 kDa
GenBank accession numberBC000985
Gene symbol SOX15
Gene ID (NCBI) 6665
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOX15, also named as SOX12, SOX20, is a 223 amino acid protein, which contains one HMG box DNA-binding domain. SOX15 is widely expressed in fetal and adult tissues and highest level is found in fetal spinal cord and adult brain and testis. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling. Sox15 is required for skeletal muscle regeneration and is up regulated in the embryonic mouse testis.

Protocols

Product Specific Protocols
IHC protocol for SOX15 antibody 25415-1-APDownload protocol
IF protocol for SOX15 antibody 25415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols