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SOX15 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 25415-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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SOX15 Antibody WB mouse skeletal muscle tissue 25415-1-AP

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25415-1-AP( SOX15 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night

SOX15 Antibody IHC mouse embryo tissue 25415-1-AP

Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 25415-1-AP( SOX15 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

SOX15 Antibody IF mouse embryo tissue 25415-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse embryo tissue using 25415-1-AP(SOX15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected in:
mouse embryo tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse embryo tissue
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SOX15 fusion protein Ag22032
Full name:
SRY (sex determining region Y)-box 15
Calculated molecular weight:
25 kDa
Observed molecular weight:
30 kDa
GenBank accession number:
BC000985
Gene ID (NCBI):
6665
Gene symbol
SOX15
Synonyms
Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27

Background

SOX15, also named as SOX12, SOX20, is a 223 amino acid protein, which contains one HMG box DNA-binding domain. SOX15 is widely expressed in fetal and adult tissues and highest level is found in fetal spinal cord and adult brain and testis. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling. Sox15 is required for skeletal muscle regeneration and is up regulated in the embryonic mouse testis.


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