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SPTBN1 Polyclonal antibody

SPTBN1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25681-1-AP

Synonyms

Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SPTBN1 Polyclonal antibody (25681-1-AP)

    WB analysis of HEK-293 using 25681-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPTBN1 Polyclonal antibody (25681-1-AP)

    WB analysis using 25681-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using SPTBN1 Polyclonal antibody (25681-1-AP)

    WB analysis of mouse heart using 25681-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPTBN1 Polyclonal antibody (25681-1-AP)

    IF Staining of HEK-293 using 25681-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25681-1-AP (SPTBN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCaco-2 cells, mouse heart tissue, HEK-293 cells, SH-SY5Y cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25681-1-AP targets SPTBN1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPTBN1 fusion protein Ag22459
Full Name spectrin, beta, non-erythrocytic 1
Calculated Molecular Weight 2364 aa, 275 kDa
Observed Molecular Weight 275 kDa
GenBank Accession NumberBC137282
Gene Symbol SPTBN1
Gene ID (NCBI) 6711
RRIDAB_2880191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.

Protocols

Product Specific Protocols
WB protocol for SPTBN1 antibody 25681-1-APDownload protocol
IF protocol for SPTBN1 antibody 25681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols