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P62/SQSTM1 Polyclonal antibody

P62/SQSTM1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55274-1-AP

Synonyms

P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HepG2 cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue, mouse kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55274-1-AP targets P62/SQSTM1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sequestosome 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_003900
Gene Symbol SQSTM1
Gene ID (NCBI) 8878
RRIDAB_11182278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.

Protocols

Product Specific Protocols
WB protocol for P62/SQSTM1 antibody 55274-1-APDownload protocol
IHC protocol for P62/SQSTM1 antibody 55274-1-APDownload protocol
IF protocol for P62/SQSTM1 antibody 55274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism.

Authors - Shruti Singla
humanWB

Cell Death Differ

CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.

Authors - Yina Liao
humanWB

Autophagy

An integrative multi-omics approach uncovers the regulatory role of CDK7 and CDK4 in autophagy activation induced by silica nanoparticles.

Authors - Chen Ruan
mouseWB,IF

Front Pharmacol

Morroniside Inhibits H2O2-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy In Vitro.

Authors - Xue Gao
human,mouseWB

Front Pharmacol

Resolvin D1 attenuates CCl4 Induced Liver Fibrosis by Inhibiting Autophagy-Mediated HSC activation via AKT/mTOR Pathway

Authors - Jiahuan Li
mouseWB

Nutrients

Punicalagin Prevents Inflammation in LPS-Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway.

Authors - Yuan Cao