|Positive WB detected in||HEK-293 cells, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, mouse brain tissue, mouse kidney tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55274-1-AP targets P62/SQSTM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||sequestosome 1|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||NM_003900|
|Gene ID (NCBI)||8878|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.
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