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P62/SQSTM1 Polyclonal antibody

P62/SQSTM1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 55274-1-AP


sequestosome 1

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue, mouse kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55274-1-AP targets P62/SQSTM1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberNM_003900
Gene symbol SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.


Product Specific Protocols
WB protocol for P62/SQSTM1 antibody 55274-1-APDownload protocol
IHC protocol for P62/SQSTM1 antibody 55274-1-APDownload protocol
IF protocol for P62/SQSTM1 antibody 55274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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