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P62/SQSTM1 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 55274-1-AP

Featured Product KD/KO validated

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Con: 65 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

P62/SQSTM1 Antibody WB HEK-293 cells 55274-1-AP

WB result of P62/SQSTM1 antibody (55274-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells

P62/SQSTM1 Antibody WB MCF-7 cells 55274-1-AP

MCF7 cells were subjected to SDS PAGE followed by western blot with 55274-1-AP(SQSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

P62/SQSTM1 Antibody IF HepG2 cells 55274-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 55274-1-AP(P62/SQSTM1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

P62/SQSTM1 Antibody IHC human breast cancer tissue 55274-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 55274-1-AP(SQSTM1 antibody) at dilution of 1:50 (under 40x lens)

P62/SQSTM1 Antibody IHC human breast cancer tissue 55274-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 55274-1-AP(SQSTM1 antibody) at dilution of 1:50 (under 10x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
MCF7 cells, HeLa cells, MCF-7 cells, HepG2 cells, HEK-293 cells
Positive IHC detected in:
human breast cancer tissue, mouse brain tissue, mouse kidney tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
sequestosome 1
Calculated molecular weight:
48 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
NM_003900
Gene ID (NCBI):
8878
Gene symbol
SQSTM1
Synonyms
P62, sequestosome 1, SQSTM1

Background

SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.


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