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ST6GAL1 Polyclonal antibody

Name: ST6GAL1 Polyclonal antibody
Brand: Proteintech
SKU: 14355-1-AP
Rating: 4.00 (5 reviews)

ST6GAL1 Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

Reviews (5)

Cat no : 14355-1-AP

Synonyms

Alpha 2,6 ST 1, B cell antigen CD75, CD75, Sialyltransferase 1, SIAT1, ST6Gal I, ST6GAL1, ST6GalI, ST6N

Validation Data Gallery

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AllWBIPIHCFC

WB analysis of HepG2 using 14355-1-AP
WB result of ST6GAL1 antibody (14355-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ST6GAL1 transfected HepG2 cells.
WB analysis using 14355-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 14355-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 14355-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 14355-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14355-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HuH-7 using 14355-1-AP
HuH-7 cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14355-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14355-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14355-1-AP (ST6GAL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 14355-1-AP
IP result of anti-ST6GAL1 (IP:14355-1-AP, 4ug; Detection:14355-1-AP 1:600) with Raji cells lysate 2800ug.
IHC staining of human skin using 14355-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 14355-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14355-1-AP (ST6GAL1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human ovary tumor using 14355-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 14355-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14355-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14355-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14355-1-AP (ST6GAL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of Raji using 14355-1-AP
1X10^6 Raji cells were intracellularly stained with 0.2 ug Anti-Human ST6GAL1 (14355-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Cat No. 14355-1-AP

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Tested Applications

Positive WB detected in	Ramos cells, Jurkat cells, mouse liver tissue, mouse spleen tissue, Raji cells, HepG2 cells, HuH-7 cells, U-937 cells, rat liver tissue
Positive IP detected in	Raji cells
Positive IHC detected in	human skin tissue, human colon cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	Raji cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 9 publications below
IHC	See 6 publications below
IF	See 2 publications below

Product Information

14355-1-AP targets ST6GAL1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ST6GAL1 fusion protein Ag5705
Full Name	ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	43-45 kDa, 50-70 kDa
GenBank Accession Number	BC040009
Gene Symbol	ST6GAL1
Gene ID (NCBI)	6480
RRID	AB_2194422
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta, and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with glycosylation modification. (PMID: 15049997, 23358684)

Protocols

Product Specific Protocols
WB protocol for ST6GAL1 antibody 14355-1-AP	Download protocol
IHC protocol for ST6GAL1 antibody 14355-1-AP	Download protocol
IP protocol for ST6GAL1 antibody 14355-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IF	Immunity Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma Authors - Rui-Qi Wu View Article
human,mouse	WB,IHC	Int J Cancer Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway. Authors - Qingmin Yuan KD Validated View Article
human,mouse	IHC,IF	Mol Cell Proteomics Integrated Systems Analysis of the Murine and Human Pancreatic Cancer Glycomes Reveals a Tumor-Promoting Role for ST6GAL1 Authors - Emma Kurz KO Validated View Article
human	WB	Front Oncol High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells. Authors - Jun Wang KD Validated View Article
human	WB	Am J Cancer Res α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC). Authors - Fengzhou Li View Article
rat	WB	Int J Food Sci Nutr Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats Authors - Bo Peng View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Vanessa (Verified Customer) (01-12-2024)	1:1000 dilution of the primary antibody accompanied by the recommended blocking, washing and incubation steps resulted in crisp bands with no background. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Jurkat cells
FH Maggie (Verified Customer) (11-15-2023)	Overnight incubation Applications: Immunofluorescence Primary Antibody Dilution: 1:200 Cell Tissue Type: PC3 cells ST6GAL1 Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:14355-1-AP)
FH Faezeh (Verified Customer) (01-29-2023)	It showed two bands in A549 and PANC1 cell lines, we validated that top band is the correct one using siRNA against ST6GAL1. Applications: Western Blot Primary Antibody Dilution: 1:1000 ST6GAL1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:14355-1-AP)
FH Dawn (Verified Customer) (02-22-2022)	It is difficult to find good antibodies for glycoslytransferase genes, but this is a good one. It works in multiple cell lines as well. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: A549 cells - lysate after miRNA transfection. ST6GAL1 Antibody Western Blot validation (1:1000 dilution) in A549 cells - lysate after miRNA transfection. (Cat no:14355-1-AP)
FH Patricia (Verified Customer) (02-25-2020)	Did not work- even for positive control Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Ovarian cancer

ST6GAL1 Polyclonal antibody

ST6GAL1 Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Validation Data Gallery

WB analysis of HepG2 using 14355-1-AP

WB analysis using 14355-1-AP

WB analysis of mouse spleen using 14355-1-AP

WB analysis of Raji using 14355-1-AP

WB analysis of U-937 using 14355-1-AP

WB analysis of HepG2 using 14355-1-AP

WB analysis of HuH-7 using 14355-1-AP

WB analysis of Jurkat using 14355-1-AP

WB analysis of mouse liver using 14355-1-AP

IP experiment of Raji using 14355-1-AP

IHC staining of human skin using 14355-1-AP

IHC staining of human skin using 14355-1-AP

IHC staining of human ovary tumor using 14355-1-AP

IHC staining of human ovary tumor using 14355-1-AP

IHC staining of human colon cancer using 14355-1-AP

IHC staining of human colon cancer using 14355-1-AP

FC experiment of Raji using 14355-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma

Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.

Integrated Systems Analysis of the Murine and Human Pancreatic Cancer Glycomes Reveals a Tumor-Promoting Role for ST6GAL1

High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.

α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC).

Ellagic acid alleviates TNBS-induced intestinal barrier dysfunction by regulating mucin secretion and maintaining tight junction integrity in rats

Reviews